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对R100.1质粒r决定簇携带的耐药基因进行定位。

Mapping of the drug resistance genes carried by the r-determinant of the R100.1 plasmid.

作者信息

Lane D, Chandler M

出版信息

Mol Gen Genet. 1977 Nov 29;157(1):17-23. doi: 10.1007/BF00268682.

DOI:10.1007/BF00268682
PMID:340913
Abstract

We have cloned the EcoRI fragments of pLC1, a circular DNA element found in an Escherichia coli dnaAts strain integratively suppressed by R100.1 (Chandler et al., 1977a), using the plasmid vector pCR1. All the resistance genes known to be present on the r-determinant of R100.1 were found to be present on pLC1. The isolation of pCR1 derivatives carrying various EcoRI fragments of either pLC1 or R100.1 has allowed a more precise mapping of the position of the resistance genes on the R100.1 molecule.

摘要

我们使用质粒载体pCR1克隆了pLC1的EcoRI片段，pLC1是一种环状DNA元件，存在于被R100.1整合抑制的大肠杆菌dnaAts菌株中（钱德勒等人，1977年a）。已知存在于R100.1的r决定簇上的所有抗性基因都在pLC1上被发现。携带pLC1或R100.1各种EcoRI片段的pCR1衍生物的分离，使得能够更精确地定位R100.1分子上抗性基因的位置。

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Mol Gen Genet. 1977 Nov 29;157(1):17-23. doi: 10.1007/BF00268682.
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本文引用的文献

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Dissociation and reassociation of RTF and r-determinants of the R-factor NR1 in Proteus mirabilis.奇异变形杆菌中R因子NR1的RTF和r决定簇的解离与重新结合
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Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
质粒介导的细菌对抗菌药物和有毒金属离子的耐药性
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Resistance to fusidic acid in Escherichia coli mediated by the type I variant of chloramphenicol acetyltransferase. A plasmid-encoded mechanism involving antibiotic binding.由氯霉素乙酰转移酶I型变体介导的大肠杆菌对夫西地酸的耐药性。一种涉及抗生素结合的质粒编码机制。
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Dissection of the r-determinant of the plasmid R100.1: the sequence at the extremities of Tn21.质粒R100.1的R决定簇剖析：Tn21两端的序列
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Mol Gen Genet. 1980 Jan;177(2):261-70. doi: 10.1007/BF00267437.
9
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Microbiol Rev. 1988 Dec;52(4):433-51. doi: 10.1128/mr.52.4.433-451.1988.
10
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