来自大肠杆菌的脱氧核糖核酸-包膜复合物。一种复合物特异性蛋白及其对复合物稳定性的可能作用。
Deoxyribonucleic acid-envelope complexes from Escherichia coli. A complex-specific protein and its possible function for the stability of the complex.
作者信息
Heidrich H G, Olsen W L
出版信息
J Cell Biol. 1975 Nov;67(2PT.1):444-60. doi: 10.1083/jcb.67.2.444.
Abstract
The different Escherichia coli envelope fractions (cell wall, cytoplasmic membrane, and DNA-envelope complex fragments) were isolated by free-flow electrophoresis and analyzed by sodium dodecylsulfate-acrylamide gel electrophoresis. The DNA-envelope complex fragments possess a specific protein (mol wt 80,000-90,000). Upon treatment with trypsin, this protein disappears and the complex breaks down, thus releasing DNA, cell wall, and cytoplasmic membrane. Disaggregation of the complex can also be achieved by high salt concentrations. Lysozyme treatment dissolves the murein layer within the complex but does not disaggregate the complex. From these and other results on the stability of the DNA-envelope complex, conclusions can be drawn about the possible linkage within the described envelope particles.
摘要
通过自由流动电泳分离不同的大肠杆菌包膜组分（细胞壁、细胞质膜和DNA-包膜复合片段），并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分析。DNA-包膜复合片段具有一种特定蛋白质（分子量80,000 - 90,000）。用胰蛋白酶处理后，这种蛋白质消失，复合物分解，从而释放出DNA、细胞壁和细胞质膜。高盐浓度也可使复合物解聚。溶菌酶处理可溶解复合物中的胞壁质层，但不会使复合物解聚。根据这些以及关于DNA-包膜复合物稳定性的其他结果，可以得出关于所述包膜颗粒内可能连接方式的结论。
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