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通过自由流动电泳从大肠杆菌中分离的脱氧核糖核酸-包膜复合物：生化及电子显微镜表征

Deoxyribonucleic acid-envelope complexes isolated from Escherichia coli by free-flow electrophoresis: biochemical and electron microscope characterization.

作者信息

Olsen W L, Heidrich H G, Hannig K, Hofschneider P H

出版信息

J Bacteriol. 1974 May;118(2):646-53. doi: 10.1128/jb.118.2.646-653.1974.

DOI:10.1128/jb.118.2.646-653.1974
PMID:4597456
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC246800/
Abstract

A procedure for the preparative isolation of Escherichia coli cell wall, membrane, and deoxyribonucleic acid (DNA)-envelope complex fragments has been developed. The envelope fragments were produced by controlled mechanical cell breakage and isolated by density gradient centrifugation and subsequent preparative free-flow electrophoresis. The DNA-envelope complex fragments were shown to contain biochemical markers of both the cell wall and the membrane and by electron microscopy to be cell envelope fragments containing wall/membrane adhesion zones.

摘要

已开发出一种用于制备性分离大肠杆菌细胞壁、细胞膜和脱氧核糖核酸（DNA）-包膜复合片段的方法。包膜片段通过可控的机械细胞破碎产生，并通过密度梯度离心和随后的制备性自由流电泳进行分离。DNA-包膜复合片段显示含有细胞壁和细胞膜的生化标志物，并且通过电子显微镜观察表明是含有壁/膜粘附区的细胞包膜片段。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ece/246800/4d884f1bce32/jbacter00341-0345-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ece/246800/6ddfceab9323/jbacter00341-0344-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ece/246800/4d884f1bce32/jbacter00341-0345-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ece/246800/6ddfceab9323/jbacter00341-0344-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ece/246800/4d884f1bce32/jbacter00341-0345-a.jpg

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本文引用的文献

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Limitations of the phenazine methosulphate assay for succinic and related dehydrogenases.吩嗪硫酸甲酯法测定琥珀酸及相关脱氢酶的局限性。
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DNA replication studied by a new method for the isolation of cell membrane-DNA complexes.通过一种分离细胞膜 - DNA 复合物的新方法对 DNA 复制进行研究。
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Isolation of a replication origin complex from Escherichia coli.从大肠杆菌中分离复制起始复合物。
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Isolation of DNA-membrane complex in Bacillus subtilis.枯草芽孢杆菌中DNA-膜复合物的分离
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Separation of Escherichia coli penicillin-binding proteins into different membrane vesicles by agarose electrophoresis and sizing chromatography.通过琼脂糖电泳和尺寸排阻色谱法将大肠杆菌青霉素结合蛋白分离到不同的膜泡中。
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Inhibition of DNA replication initiation by aminoglycoside antibiotics.氨基糖苷类抗生素对DNA复制起始的抑制作用。
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8
Deoxyribonucleic acid-envelope complexes from Escherichia coli. A complex-specific protein and its possible function for the stability of the complex.来自大肠杆菌的脱氧核糖核酸-包膜复合物。一种复合物特异性蛋白及其对复合物稳定性的可能作用。
J Cell Biol. 1975 Nov;67(2PT.1):444-60. doi: 10.1083/jcb.67.2.444.
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Characterization of the cytoplasmic fibrils of Treponema refringens (Nichols).致密螺旋体（尼科尔斯株）胞质纤丝的特性研究
Arch Microbiol. 1979 Mar 12;120(3):205-14. doi: 10.1007/BF00423067.
10
Altered accumulation of a membrane protein unique to a membrane-deoxyribonucleic acid complex in a dna initiation mutant of Bacillus subtilis.枯草芽孢杆菌DNA起始突变体中膜-脱氧核糖核酸复合物特有的一种膜蛋白积累的改变。
J Bacteriol. 1977 Jun;130(3):1224-33. doi: 10.1128/jb.130.3.1224-1233.1977.
J Bacteriol. 1971 Oct;108(1):545-52. doi: 10.1128/jb.108.1.545-552.1971.
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Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳对大肠杆菌细胞膜的蛋白质组成进行检测。
J Bacteriol. 1970 Nov;104(2):882-9. doi: 10.1128/jb.104.2.882-889.1970.
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Adsorption of bacteriophages to adhesions between wall and membrane of Escherichia coli.噬菌体对大肠杆菌细胞壁与细胞膜间粘连处的吸附作用。
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Separation by density gradient centrifugation of two types of membranes from spheroplast membrane of Escherichia coli K12.通过密度梯度离心法从大肠杆菌K12原生质球膜中分离出两种类型的膜。
Biochim Biophys Acta. 1968 Jan 3;150(1):159-61. doi: 10.1016/0005-2736(68)90020-5.
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Annu Rev Biochem. 1972;41(10):301-32. doi: 10.1146/annurev.bi.41.070172.001505.
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Distribution of lipids in the wall and cytoplasmic membrane subfractions of the cell envelope of Escherichia coli.脂质在大肠杆菌细胞包膜的细胞壁和细胞质膜亚组分中的分布。
J Bacteriol. 1972 Feb;109(2):686-90. doi: 10.1128/jb.109.2.686-690.1972.
10
The topography of the bacterial cell wall.细菌细胞壁的拓扑结构。
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