鉴定一种涉及大肠杆菌复制起点的具有生化独特性的DNA-膜相互作用。
Identification of a biochemically unique DNA-membrane interaction involving the Escherichia coli origin of replication.
作者信息
Craine B L, Rupert C S
出版信息
J Bacteriol. 1978 Apr;134(1):193-9. doi: 10.1128/jb.134.1.193-199.1978.
Abstract
DNA-membrane complexes have been obtained from Escherichia coli by using a freeze-thaw lysis procedure that avoids lysozyme and detergents. Complexes made in this manner and containing DNA near the origin of replication are uniquely sensitive to ionic strength, Pronase, and trypsin. There is approximately one such complex per chromosomal origin. The sensitivities suggest that origin-specific binding is mediated by a protein. By using these unique characteristics to distinguish origin-specific complexes from the majority of DNA-membrane binding sites, it was found that the origin-specific binding persists after termination of chromosomal replication.
摘要
通过使用避免使用溶菌酶和去污剂的冻融裂解程序，从大肠杆菌中获得了DNA-膜复合物。以这种方式制备的、在复制起点附近含有DNA的复合物对离子强度、链霉蛋白酶和胰蛋白酶具有独特的敏感性。每个染色体起点大约有一个这样的复合物。这些敏感性表明，起点特异性结合是由一种蛋白质介导的。利用这些独特的特性将起点特异性复合物与大多数DNA-膜结合位点区分开来，发现染色体复制终止后，起点特异性结合仍然存在。
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本文引用的文献
1
Association between the chromosome and the cytoplasmic membrane in Bacillus subtilis.枯草芽孢杆菌中染色体与细胞质膜之间的关联。
Biochem Biophys Res Commun. 1969 May 8;35(3):354-62. doi: 10.1016/0006-291x(69)90506-3.
2
Membrane attachment of the chromosome replication origin in Bacillus subtilis.枯草芽孢杆菌中染色体复制起点的膜附着
Cold Spring Harb Symp Quant Biol. 1968;33:695-705. doi: 10.1101/sqb.1968.033.01.078.
3
Escherichia coli mutants temperature-sensitive for DNA synthesis.对DNA合成温度敏感的大肠杆菌突变体。
Mol Gen Genet. 1971;113(3):273-84. doi: 10.1007/BF00339547.
4
Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli.大肠杆菌复制起点处DNA与细胞膜稳定附着的证据。
Biochem Biophys Res Commun. 1970 Oct 9;41(1):157-62. doi: 10.1016/0006-291x(70)90482-1.
5
Properties of the growing point region in the bacterial chromosome.细菌染色体中生长点区域的特性
Biochim Biophys Acta. 1967 Dec 19;149(2):519-31. doi: 10.1016/0005-2787(67)90180-3.
6
Chromosome replication and the division cycle of Escherichia coli B/r.大肠杆菌B/r的染色体复制与分裂周期
J Mol Biol. 1968 Feb 14;31(3):519-40. doi: 10.1016/0022-2836(68)90425-7.
7
The effects of lysozyme on DNA--membrane association in Escherichia coli.溶菌酶对大肠杆菌中DNA与膜结合的影响。
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8
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9
Chromosomal sites of DNA-membrane attachment in Escherichia coli.大肠杆菌中DNA与膜附着的染色体位点。
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Protein synthesis and the release of the replicated chromosome from the cell membrane.蛋白质合成以及复制后的染色体从细胞膜上释放。
Nature. 1974 Sep 20;251(5472):252-4. doi: 10.1038/251252a0.
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