鉴定一种涉及大肠杆菌复制起点的具有生化独特性的DNA-膜相互作用。
Identification of a biochemically unique DNA-membrane interaction involving the Escherichia coli origin of replication.
作者信息
Craine B L, Rupert C S
出版信息
J Bacteriol. 1978 Apr;134(1):193-9. doi: 10.1128/jb.134.1.193-199.1978.
Abstract
DNA-membrane complexes have been obtained from Escherichia coli by using a freeze-thaw lysis procedure that avoids lysozyme and detergents. Complexes made in this manner and containing DNA near the origin of replication are uniquely sensitive to ionic strength, Pronase, and trypsin. There is approximately one such complex per chromosomal origin. The sensitivities suggest that origin-specific binding is mediated by a protein. By using these unique characteristics to distinguish origin-specific complexes from the majority of DNA-membrane binding sites, it was found that the origin-specific binding persists after termination of chromosomal replication.
摘要
通过使用避免使用溶菌酶和去污剂的冻融裂解程序，从大肠杆菌中获得了DNA-膜复合物。以这种方式制备的、在复制起点附近含有DNA的复合物对离子强度、链霉蛋白酶和胰蛋白酶具有独特的敏感性。每个染色体起点大约有一个这样的复合物。这些敏感性表明，起点特异性结合是由一种蛋白质介导的。利用这些独特的特性将起点特异性复合物与大多数DNA-膜结合位点区分开来，发现染色体复制终止后，起点特异性结合仍然存在。
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