体外促进克氏锥虫（恰加斯锥虫）摄取[³H]胸腺嘧啶核苷的血清蛋白。

Serum proteins promoting [3H]thymidine uptake by Trypanosoma (Schizotrypanum) cruzi (Chagas) in vitro.

作者信息

O'Daly J A

出版信息

J Protozool. 1975 Nov;22(4):550-5. doi: 10.1111/j.1550-7408.1975.tb05231.x.

DOI:10.1111/j.1550-7408.1975.tb05231.x

Abstract

Five proteins capable of stimulating [3H]thymidine uptake by Trypanosoma cruzi in vitro were isolated from fetal calf serum by (NH4)2SO4 precipitation and ion exchange column chromatography. The proteins were partially characterized by immunodiffusion, immunoelectrophoresis, polyacrylamide gel disc electrophoresis, and SDS electrophoresis. As estimated by SDS electrophoresis, using 4 standards, the molecular weight of protein 1 was 100,000, that of protein 2 was 76,000, and that of proteins 3-5 was 68,000 daltons.

摘要

通过硫酸铵沉淀和离子交换柱色谱法从胎牛血清中分离出五种能够在体外刺激克氏锥虫摄取[3H]胸苷的蛋白质。通过免疫扩散、免疫电泳、聚丙烯酰胺凝胶圆盘电泳和SDS电泳对这些蛋白质进行了部分表征。使用4种标准品通过SDS电泳估计，蛋白质1的分子量为100,000，蛋白质2的分子量为76,000，蛋白质3 - 5的分子量为68,000道尔顿。