O'Daly J A
J Protozool. 1976 Nov;23(4):577-83. doi: 10.1111/j.1550-7408.1976.tb03845.x.
Division and epimastigote-to-trypomastigote transformation of Trypanosoma cruzi were observed in O'Daly's SM medium supplemented, in place of whole fetal calf serum, with fractions of this serum, its partially purified proteins, or with mixtures of these fractions and proteins. In addition to their division-promoting effects, most but not all serum fractions stimulated [3H]thymidine uptake by the flagellates. As revealed by TEAE-cellulose column chromatography and immunoelectrophoresis, the serum fractions were altered during the logarithmic growth phase of the trypanosomes.
在奥代氏SM培养基中观察到克氏锥虫的分裂以及上鞭毛体向锥鞭毛体的转变,该培养基用血清的部分成分、其部分纯化的蛋白质或这些成分与蛋白质的混合物替代了全胎牛血清。除了具有促进分裂的作用外,大多数(但并非全部)血清成分刺激了鞭毛虫对[³H]胸腺嘧啶核苷的摄取。经二乙氨基乙基纤维素柱层析和免疫电泳显示,血清成分在锥虫的对数生长期发生了变化。