Ramírez M, Alejandra O, Ortiz de Bertorelli L
Facultad de Agronomía, Universidad Central de Venezuela.
Arch Latinoam Nutr. 2000 Mar;50(1):69-73.
The purpose of this work was the isolation and characterization of grain protein from five Venezuelan Genotypes (U-02, Yaracuy, Valle De La Pascua, Originally Tovar) of Jack Bean (Canavalia ensiformis). The average protein content from these genotypes was 31.37%, it ranged between 28.44% and 33.05%. The protein isolation was performed by solubility extraction procedures, showed: 84.57% of albumins, globulins and non proteic nitrogen and 15.43% of alcohol insoluble reduced glutelin (AIG). The content of anti-nutritional factors (canavanine and hemagglutination title) found in protein fractions were respectively: Albumins 1.96%, +4; globulins 0.17%, +5 and AIG 0.22%, +1. It was observed that protein fractions of genotype Tovar had the lowest canavanine values (0.79%, 0.02% and 0.00% respectively). The globulins gave the highest in vitro protein digestibility (65.20%) followed by Albumins (58.90%) and AIG (37.28%).
这项工作的目的是从五种委内瑞拉基因型(U-02、亚拉库伊、帕斯夸山谷、原托瓦尔)的金甲豆(Canavalia ensiformis)中分离并鉴定谷物蛋白。这些基因型的平均蛋白质含量为31.37%,范围在28.44%至33.05%之间。蛋白质分离通过溶解度提取程序进行,结果显示:白蛋白、球蛋白和非蛋白氮占84.57%,醇不溶性还原谷蛋白(AIG)占15.43%。在蛋白质组分中发现的抗营养因子(刀豆氨酸和血凝效价)含量分别为:白蛋白1.96%,+4;球蛋白0.17%,+5;AIG 0.22%,+1。观察到托瓦尔基因型的蛋白质组分刀豆氨酸值最低(分别为0.79%、0.02%和0.00%)。球蛋白的体外蛋白质消化率最高(65.20%),其次是白蛋白(58.90%)和AIG(37.28%)。