Zvyagintseva T N, Shevchenko N M, Nazarova I V, Scobun A S, Luk'yanov P A, Elyakova L A
Laboratory of Enzymatic Chemistry, Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok.
Comp Biochem Physiol C Toxicol Pharmacol. 2000 Jul;126(3):209-15. doi: 10.1016/s0742-8413(00)00114-6.
Fucoidans and laminarans from Laminaria cichorioides, Laminaria japonica, Fucus evanescens, laminaran from Laminaria gurjanovae, other beta-D-glucans (translam, pustulan and zymosan) and lambda-carrageenan from Chondrus armatus were used to study the effect of water-soluble polysaccharides from seaweeds on the alternative pathway of complement (APC). beta-D-Glucans and fucoidans under study differed appreciably from each other by structural characteristics, and also by degree of purification. beta-D-glucans, on ability to bind complement, ranked in a line according to a degree of their purification. Highly purified beta-D-glucans under study did not reveal an ability to bind complement. The fucoidans were divided conventionally into three groups according to their action on APC. Highly sulfated alpha-L-fucan from L. cichorioides with the greatest activity toward APC and caused 50% inhibition of reaction of activation (RA) of APC in a concentration of 0.5-0.7 mg/ml. Opposite 50% of inhibition of lysis of erythrocytes by sulfated heterogeneous fucoidan from L. japonica was achieved with 20 mg/ml. All other fucoidans and lambda-carrageenan have activity at 6-10 mg/ml concentration. Decreasing the sulfate content from 36% up to 9% in sample fucoidans under study was not reflected practically in the 50% inhibition concentration. Apparently, the degree of sulfating of fucoidans did not influence their action on APC. But the positive influence of fucose in structure of polysaccharide was obvious.
采用来自菊苣海带、海带、消失墨角藻的岩藻聚糖和海带多糖、古氏海带的海带多糖、其他β-D-葡聚糖（转糖醛酸、支链淀粉和酵母聚糖）以及来自角叉菜的λ-卡拉胶，研究海藻水溶性多糖对补体替代途径（APC）的影响。所研究的β-D-葡聚糖和岩藻聚糖在结构特征以及纯化程度上存在明显差异。β-D-葡聚糖在结合补体的能力方面，根据其纯化程度呈线性排列。所研究的高度纯化的β-D-葡聚糖未显示出结合补体的能力。根据岩藻聚糖对APC的作用，通常将其分为三组。来自菊苣海带的高度硫酸化的α-L-岩藻聚糖对APC具有最大活性，在浓度为0.5 - 0.7 mg/ml时可导致APC激活反应（RA）的50%抑制。相反，来自海带的硫酸化异质岩藻聚糖在浓度为20 mg/ml时可实现对红细胞裂解的50%抑制。所有其他岩藻聚糖和λ-卡拉胶在浓度为6 - 10 mg/ml时具有活性。在所研究的样品岩藻聚糖中，将硫酸酯含量从36%降至9%实际上并未反映在50%抑制浓度上。显然，岩藻聚糖的硫酸化程度并未影响其对APC的作用。但多糖结构中岩藻糖的积极影响是明显的。
