Shevchenko N M, Anastiuk S D, Gerasimenko N I, Dmitrenok P S, Isakov V V, Zviagintseva T N
Bioorg Khim. 2007 Jan-Feb;33(1):96-107. doi: 10.1134/s1068162007010116.
Polysaccharide and lipid composition of the Pacific brown seaweed Laminaria gurjanovae is determined. Alginic acid is shown to be the main polysaccharide of its biomass (about 28%); it consists of mannuronic and guluronic acid residues at a ratio of 3 : 1. The yield of water-soluble polymannuronic acid is low and does not exceed 1.1% of dry biomass. High laminaran content (about 22%) is found, whereas the yield of fucoidan is no more than 3.6%. Laminaran consists of two fractions, soluble and insoluble in cold water, their ratio is 2.5 : 1. Practically, insoluble laminaran is a linear 1,3-beta-D-glucan, and the soluble fraction was shown to be 1,3;1,6- 3-D-glucan. The oligosaccharide products of desulfation or partial acidic hydrolysis of fucoidan were studied by MALDI TOF MS; they were found to be fuco- and galactooligosaccharides. The fucoidan is suggested to be a highly sulfated partially acetylated galactofucan (Fuc/Gal is -1 : 1). The main lipid components of the dried L. gurjanovae are neutral lipids and glyceroglycolipids, whereas phospholipids are found in minor amounts. The main fatty acid components of lipids are 14 : 0, 16 : 0, 16 : 1 n-7, 18 : 1 n-7, and 18 : 2 n-6 acids.
测定了太平洋褐藻古氏海带的多糖和脂质组成。结果表明，海藻酸是其生物量中的主要多糖（约28%）；它由甘露糖醛酸和古洛糖醛酸残基以3:1的比例组成。水溶性聚甘露糖醛酸的产量较低，不超过干生物量的1.1%。发现海带多糖含量较高（约22%），而岩藻依聚糖的产量不超过3.6%。海带多糖由可溶于冷水和不溶于冷水的两个部分组成，其比例为2.5:1。实际上，不溶性海带多糖是一种线性1,3-β-D-葡聚糖，而可溶部分显示为1,3;1,6-3-D-葡聚糖。通过基质辅助激光解吸电离飞行时间质谱（MALDI TOF MS）研究了岩藻依聚糖的脱硫或部分酸性水解的寡糖产物；发现它们是岩藻寡糖和半乳寡糖。岩藻依聚糖被认为是一种高度硫酸化的部分乙酰化的半乳岩藻聚糖（岩藻糖/半乳糖为-1:1）。干燥的古氏海带的主要脂质成分是中性脂质和甘油糖脂，而磷脂含量较少。脂质的主要脂肪酸成分是14:0、16:0、16:1 n-7、18:1 n-7和18:2 n-6酸。
