Mitchell R J, Federle L, Sofro A S, Papiha S S, Briceno I, Bernal J E
Department of Genetics and Human Variation, La Trobe University, Bundoora, Victoria, Australia.
Hum Biol. 2000 Aug;72(4):697-705.
We report the frequencies of alleles at the microsatellite locus D12S67 in 2 widely separated ethnic groups of the world: 2 populations from Sulawesi, an island in the Indonesian archipelago, and 5 Native American tribes of Colombia, South America. The allele frequencies in the Minihasans and Torajans of Sulawesi are similar to each other (but the modal class allele is different) and in general agreement with those reported in mainland Asian groups, but different from both Europeans and Chinese Han of Taiwan. The 5 Native American tribes (Arsario, Kogui, Ijka, Wayuu, and Coreguaje) display different allele frequencies from those seen in Sulawesi populations, in other groups from Europe and mainland Asia, and in Chinese Han of Taiwan. Native Americans exhibit a bimodal distribution of alleles, unlike other groups, with significant differences among the tribes. The Arsario and Kogui have no admixture with Europeans or Africans and are the most distinctive, while the Wayuu have the most admixture and show most similarity to other groups. The data suggest that nonadmixed Native Americans may be quite distinctive with respect to this marker. The most common allele varies across the 5 tribes, from 249 base pairs to 261 base pairs. All samples exhibit Hardy-Weinberg genotype proportions; heterozygosities are lowest in the 2 nonadmixed Native American tribes. Examination of all the available data indicates that some east Asian and southeast Asian groups are characterized by a high frequency of smaller sized D12S67 alleles, while other populations have a greater proportion of the larger sized alleles. The cumulative, though still highly restricted, population data on locus D12S67 demonstrate that it may be of considerable value in anthropological genetic studies of ethnic groups. Data are required on Native Americans outside Colombia before this marker can be used in admixture studies of this group.
我们报告了世界上两个相距甚远的种族群体中微卫星位点D12S67的等位基因频率：来自印度尼西亚群岛苏拉威西岛的2个群体，以及南美洲哥伦比亚的5个美洲原住民部落。苏拉威西岛的米尼哈山人和托拉雅人的等位基因频率彼此相似（但众数类等位基因不同），总体上与亚洲大陆群体中报告的频率一致，但与欧洲人和台湾汉族不同。5个美洲原住民部落（阿尔萨里奥人、科吉人、伊伊卡人、瓦尤人、科雷瓜耶人）显示出与苏拉威西岛群体、欧洲和亚洲大陆其他群体以及台湾汉族不同的等位基因频率。与其他群体不同，美洲原住民表现出等位基因的双峰分布，各部落之间存在显著差异。阿尔萨里奥人和科吉人与欧洲人或非洲人没有混合，是最独特的，而瓦尤人混合程度最高，与其他群体最相似。数据表明，未混合的美洲原住民在这个标记方面可能非常独特。最常见的等位基因在5个部落中各不相同，从249个碱基对到261个碱基对。所有样本均表现出哈迪-温伯格基因型比例；杂合度在2个未混合的美洲原住民部落中最低。对所有现有数据的检查表明，一些东亚和东南亚群体的特征是较小尺寸的D12S67等位基因频率较高，而其他群体中较大尺寸等位基因的比例更大。关于位点D12S67的累积（尽管仍然非常有限）群体数据表明，它在种族群体的人类学遗传研究中可能具有相当大的价值。在将这个标记用于该群体的混合研究之前，需要获取哥伦比亚以外美洲原住民的数据。
