Modéer T, Domeij H, Andurén I, Mustafa M, Brunius G
Department of Pediatric Dentistry, Karolinska Institutet, Huddinge, Sweden.
J Oral Pathol Med. 2000 Nov;29(10):491-9. doi: 10.1034/j.1600-0714.2000.291003.x.
The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1beta (IL-1beta), was studied. PHT (20 microg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1beta (30 pg/ml). The stimulatory effect of PHT on IL-1beta-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 microM). The anti-inflammatory drug, dexamethasone (1 microM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1beta. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth.
研究了苯妥英（PHT）在体外对人牙龈成纤维细胞中白细胞介素-6（IL-6）和白细胞介素-8（IL-8）产生的影响，这些细胞分别在有无白细胞介素-1β（IL-1β）刺激的情况下进行实验。单独使用PHT（20微克/毫升）可增加IL-6和IL-8的mRNA水平，并在转录和翻译水平上协同增强受IL-1β（30皮克/毫升）刺激的成纤维细胞中IL-6和IL-8的产生。特异性环氧化酶-2抑制剂NS-398（1微摩尔）可显著降低PHT对IL-1β诱导的IL-6产生的刺激作用。抗炎药物地塞米松（1微摩尔）可消除在有或无IL-1β情况下受PHT刺激的牙龈成纤维细胞中IL-6和IL-8的产生。PHT单独以及与IL-1联合上调人牙龈成纤维细胞中IL-6和IL-8产生的能力，可能有助于增强炎症细胞的募集和激活。PHT的这种作用可能为牙周组织中细胞因子与结缔组织细胞之间建立相互作用提供了前提条件，而这种相互作用被认为会导致牙龈增生。
