海马突触处的“亲吻并跑”式胞吐作用。
"Kiss and run" exocytosis at hippocampal synapses.
作者信息
Stevens C F, Williams J H
机构信息
Howard Hughes Medical Institute, and Molecular Neurobiology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
出版信息
Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12828-33. doi: 10.1073/pnas.230438697.
Abstract
We have combined electrophysiology and imaging to measure the release of neurotransmitter and fluorescent dye at synapses of cultured hippocampal neurons. These experiments have revealed a "kiss and run" mode of exocytosis in which synaptic vesicles release glutamate normally but do not permit dye to enter or escape from the vesicle. During "kiss and run," the vesicle interior may be exposed very transiently (<6 ms), or a special configuration of the fusion pore may prevent dye exchange. We estimate that about 20% of the vesicles normally use this "kiss and run" pathway, and that the fraction of "kiss and run" events can be increased to over 80% by superfusing the synapses with hypertonic solution.
摘要
我们结合了电生理学和成像技术来测量培养的海马神经元突触处神经递质和荧光染料的释放。这些实验揭示了一种“亲吻并跑开”的胞吐模式，即突触小泡正常释放谷氨酸，但不允许染料进入或从泡囊中逸出。在“亲吻并跑开”过程中，泡囊内部可能仅短暂暴露（<6毫秒），或者融合孔的特殊结构可能阻止染料交换。我们估计，正常情况下约20%的小泡使用这种“亲吻并跑开”途径，并且通过用高渗溶液灌流突触，“亲吻并跑开”事件的比例可增加到80%以上。
相似文献
1
"Kiss and run" exocytosis at hippocampal synapses.海马突触处的“亲吻并跑”式胞吐作用。
Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12828-33. doi: 10.1073/pnas.230438697.
2
Vesicular release mode shapes the postsynaptic response at hippocampal synapses.囊泡释放模式塑造海马突触的突触后反应。
J Physiol. 2009 Nov 1;587(Pt 21):5073-80. doi: 10.1113/jphysiol.2009.175315. Epub 2009 Sep 14.
3
Frequency-dependent kinetics and prevalence of kiss-and-run and reuse at hippocampal synapses studied with novel quenching methods.利用新型淬灭方法研究海马突触处依赖频率的动力学以及亲吻-逃离和再利用的发生率。
Neuron. 2006 Jan 19;49(2):243-56. doi: 10.1016/j.neuron.2005.12.018.
4
Real-time three-dimensional tracking of single synaptic vesicles reveals that synaptic vesicles undergoing kiss-and-run fusion remain close to their original fusion site before reuse.实时三维追踪单个突触小泡显示，经历 kiss-and-run 融合的突触小泡在重新使用之前仍接近其原始融合位点。
Biochem Biophys Res Commun. 2019 Jun 30;514(3):1004-1008. doi: 10.1016/j.bbrc.2019.05.043. Epub 2019 May 12.
5
Potassium ion- and nitric oxide-induced exocytosis from populations of hippocampal synapses during synaptic maturation in vitro.体外突触成熟过程中钾离子和一氧化氮诱导海马突触群体的胞吐作用。
Neuroscience. 1997 Oct;80(4):1057-73. doi: 10.1016/s0306-4522(97)00152-8.
6
Single synaptic vesicles fusing transiently and successively without loss of identity.单个突触小泡短暂且连续地融合，而不丧失其特性。
Nature. 2003 Jun 5;423(6940):643-7. doi: 10.1038/nature01686.
7
Three modes of synaptic vesicular recycling revealed by single-vesicle imaging.单囊泡成像揭示的突触囊泡循环的三种模式。
Nature. 2003 Jun 5;423(6940):607-13. doi: 10.1038/nature01677.
8
The synaptic vesicle cycle revisited.再探突触小泡循环。
Neuron. 2000 Nov;28(2):317-20. doi: 10.1016/s0896-6273(00)00109-4.
9
Fast vesicle recycling supports neurotransmission during sustained stimulation at hippocampal synapses.快速囊泡循环在海马突触持续刺激期间支持神经传递。
J Neurosci. 2002 Mar 1;22(5):1608-17. doi: 10.1523/JNEUROSCI.22-05-01608.2002.
10
Imaging single synaptic vesicles undergoing repeated fusion events: kissing, running, and kissing again.对经历反复融合事件的单个突触小泡进行成像：接触、移动、再接触。
Neuropharmacology. 2003 Nov;45(6):797-813. doi: 10.1016/s0028-3908(03)00310-1.
引用本文的文献
1
Dynamic regulation of vesicle pools in a detailed spatial model of the complete synaptic vesicle cycle.完整突触小泡循环精细空间模型中囊泡池的动态调节
Sci Adv. 2025 May 30;11(22):eadq6477. doi: 10.1126/sciadv.adq6477. Epub 2025 May 28.
2
Subcellular functions of tau mediate repair response and synaptic homeostasis in injury.tau蛋白的亚细胞功能介导损伤后的修复反应和突触稳态。
Mol Psychiatry. 2025 Apr 23. doi: 10.1038/s41380-025-03029-6.
3
Vesicle fusion and release in neurons under dynamic mechanical equilibrium.动态力学平衡下神经元中的囊泡融合与释放
iScience. 2024 Apr 19;27(5):109793. doi: 10.1016/j.isci.2024.109793. eCollection 2024 May 17.
4
Dysfunction of synaptic endocytic trafficking in Parkinson's disease.帕金森病中突触内吞运输功能障碍。
Neural Regen Res. 2024 Dec 1;19(12):2649-2660. doi: 10.4103/NRR.NRR-D-23-01624. Epub 2024 Mar 1.
5
Subcellular functions of tau mediates repair response and synaptic homeostasis in injury.tau蛋白的亚细胞功能介导损伤后的修复反应和突触稳态。
Res Sq. 2024 Feb 29:rs.3.rs-3897741. doi: 10.21203/rs.3.rs-3897741/v1.
6
Exosome secretion kinetics are controlled by temperature.外泌体的分泌动力学受温度控制。
Biophys J. 2023 Apr 4;122(7):1301-1314. doi: 10.1016/j.bpj.2023.02.025. Epub 2023 Feb 22.
7
Mechanisms of Synaptic Vesicle Exo- and Endocytosis.突触小泡胞吐和胞吞作用的机制。
Biomedicines. 2022 Jul 4;10(7):1593. doi: 10.3390/biomedicines10071593.
8
Synaptic Vesicle Recycling and the Endolysosomal System: A Reappraisal of Form and Function.突触小泡循环与内溶酶体系统：形式与功能的重新评估
Front Synaptic Neurosci. 2022 Feb 25;14:826098. doi: 10.3389/fnsyn.2022.826098. eCollection 2022.
9
Visualization of Partial Exocytotic Content Release and Chemical Transport into Nanovesicles in Cells.细胞中部分胞吐内容物释放和化学物质运输到纳米囊泡的可视化。
ACS Nano. 2022 Mar 22;16(3):4831-4842. doi: 10.1021/acsnano.2c00344. Epub 2022 Feb 21.
10
Frequency-Dependent Engagement of Synaptic Vesicle Pools in the Mice Motor Nerve Terminals.小鼠运动神经末梢中突触小泡池的频率依赖性参与
Cell Mol Neurobiol. 2023 Mar;43(2):729-739. doi: 10.1007/s10571-022-01202-x. Epub 2022 Feb 3.
本文引用的文献
1
Neurotransmitter release: fusion or 'kiss-and-run'?神经递质释放：融合还是“亲吻-逃离”？
Trends Cell Biol. 1994 Jan;4(1):1-4. doi: 10.1016/0962-8924(94)90025-6.
2
Transport, capture and exocytosis of single synaptic vesicles at active zones.活性区单个突触小泡的运输、捕获和胞吐作用。
Nature. 2000 Aug 24;406(6798):849-54. doi: 10.1038/35022500.
3
Peeping at the vesicle kiss.窥视囊泡融合。
Nat Cell Biol. 1999 May;1(1):E3-4. doi: 10.1038/8950.
4
High calcium concentrations shift the mode of exocytosis to the kiss-and-run mechanism.高钙浓度会将胞吐作用的模式转变为亲吻式释放机制。
Nat Cell Biol. 1999 May;1(1):40-4. doi: 10.1038/9012.
5
Reversal of synaptic vesicle docking at central synapses.中枢突触处突触小泡对接的逆转。
Nat Neurosci. 1999 Jun;2(6):503-7. doi: 10.1038/9149.
6
Monitoring secretory membrane with FM1-43 fluorescence.使用FM1-43荧光监测分泌膜。
Annu Rev Neurosci. 1999;22:1-10. doi: 10.1146/annurev.neuro.22.1.1.
7
Augmentation is a potentiation of the exocytotic process.增强是胞吐过程的一种强化作用。
Neuron. 1999 Jan;22(1):139-46. doi: 10.1016/s0896-6273(00)80685-6.
8
The kinetics of exocytosis and endocytosis in the synaptic terminal of goldfish retinal bipolar cells.金鱼视网膜双极细胞突触终末的胞吐作用和胞吞作用动力学
J Physiol. 1999 Feb 15;515 ( Pt 1)(Pt 1):181-202. doi: 10.1111/j.1469-7793.1999.181ad.x.
9
Activity-dependent modulation of the rate at which synaptic vesicles become available to undergo exocytosis.依赖活动对突触囊泡可用于进行胞吐作用的速率的调节。
Neuron. 1998 Aug;21(2):415-24. doi: 10.1016/s0896-6273(00)80550-4.
10
Kinetics and regulation of fast endocytosis at hippocampal synapses.海马突触快速内吞作用的动力学与调控
Nature. 1998 Aug 6;394(6693):581-5. doi: 10.1038/29079.
Zotero 插件