中枢突触处突触小泡对接的逆转。

Reversal of synaptic vesicle docking at central synapses.

作者信息

Murthy V N, Stevens C F

机构信息

Howard Hughes Medical Institute, and Molecular Neurobiology Lab, Salk Institute, 10010 North Torrey Pines Road, La Jolla, California 92037, USA.

出版信息

Nat Neurosci. 1999 Jun;2(6):503-7. doi: 10.1038/9149.

DOI:10.1038/9149

PMID:10448213

Abstract

We used quantitative fluorescence imaging of vesicles labeled with membrane-soluble dyes to determine rates of undocking and spontaneous exocytosis of vesicles docked to the active zone of hippocampal synapses in culture. Individual vesicles undock about once per two minutes and spontaneously exocytose about once per eight minutes. Thus, not only does undocking occur, but it is over threefold faster than spontaneous fusion.

摘要

我们利用对用膜溶性染料标记的囊泡进行定量荧光成像，来确定培养的海马突触活性区停靠的囊泡的解停靠速率和自分泌速率。单个囊泡大约每两分钟解停靠一次，大约每八分钟自分泌一次。因此，不仅存在解停靠现象，而且其速度比自发融合快三倍多。