Srikantan V, Zou Z, Petrovics G, Xu L, Augustus M, Davis L, Livezey J R, Connell T, Sesterhenn I A, Yoshino K, Buzard G S, Mostofi F K, McLeod D G, Moul J W, Srivastava S
Center for Prostate Disease Research, Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799, USA.
Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12216-21. doi: 10.1073/pnas.97.22.12216.
A prostate-specific gene, PCGEM1, was identified by differential display analysis of paired normal and prostate cancer tissues. Multiple tissue Northern blot analysis revealed that PCGEM1 was expressed exclusively in human prostate tissue. Analysis of PCGEM1 expression in matched normal and primary tumor specimens revealed tumor-associated overexpression in 84% of patients with prostate cancer by in situ hybridization assay and in 56% of patients by reverse transcription-PCR assay. Among various prostate cancer cell lines analyzed, PCGEM1 expression was detected only in the androgen receptor-positive cell line LNCaP. Extensive DNA sequence analysis of the PCGEM1 cDNA and genomic DNA revealed that PCGEM1 lacks protein-coding capacity and suggests that it may belong to an emerging class of noncoding RNAs, also called "riboregulators." The PCGEM1 locus was mapped to chromosome 2q32. Taken together, the remarkable prostate-tissue specificity and androgen-dependent expression of PCGEM1 as well as its elevated expression in a significant percentage of tumor tissues suggest specific functions of PCGEM1 in the biology and tumorigenesis of the prostate gland.
通过对配对的正常前列腺组织和前列腺癌组织进行差异显示分析，鉴定出一种前列腺特异性基因PCGEM1。多组织Northern印迹分析显示，PCGEM1仅在人前列腺组织中表达。对匹配的正常和原发性肿瘤标本中PCGEM1表达的分析表明，通过原位杂交检测，84%的前列腺癌患者中存在肿瘤相关的过表达，通过逆转录-聚合酶链反应检测，56%的患者存在过表达。在分析的各种前列腺癌细胞系中，仅在雄激素受体阳性细胞系LNCaP中检测到PCGEM1表达。对PCGEM1 cDNA和基因组DNA进行广泛的DNA序列分析表明，PCGEM1缺乏蛋白质编码能力，提示它可能属于一类新兴的非编码RNA，也称为“核糖调节因子”。PCGEM1基因座定位于2号染色体q32。综上所述，PCGEM1显著的前列腺组织特异性、雄激素依赖性表达及其在相当比例肿瘤组织中的高表达表明，PCGEM1在前列腺生物学和肿瘤发生中具有特定功能。
