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本文引用的文献

1
Molecular cloning and expression analysis of the human DA41 gene and its mapping to chromosome 9q21.2-q21.3.人类DA41基因的分子克隆与表达分析及其在9号染色体q21.2-q21.3上的定位
J Hum Genet. 2000;45(3):188-91. doi: 10.1007/s100380050209.
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Ubiquitin-related proteins regulate interaction of vimentin intermediate filaments with the plasma membrane.
Mol Cell. 1999 Oct;4(4):619-25. doi: 10.1016/s1097-2765(00)80212-9.
3
Identification of XDRP1; a Xenopus protein related to yeast Dsk2p binds to the N-terminus of cyclin A and inhibits its degradation.XDRP1的鉴定；一种与酵母Dsk2p相关的非洲爪蟾蛋白与细胞周期蛋白A的N端结合并抑制其降解。
EMBO J. 1999 Sep 15;18(18):5009-18. doi: 10.1093/emboj/18.18.5009.
4
Dissociation of CDK2 from cyclin A in response to the topoisomerase II inhibitor etoposide in v-src-transformed but not normal NIH 3T3 cells.在v-src转化而非正常的NIH 3T3细胞中，拓扑异构酶II抑制剂依托泊苷可诱导细胞周期蛋白依赖性激酶2（CDK2）与细胞周期蛋白A解离。
Exp Cell Res. 1999 Jun 15;249(2):327-36. doi: 10.1006/excr.1999.4484.
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DAN is a secreted glycoprotein related to Xenopus cerberus.DAN是一种与非洲爪蟾Xenopus cerberus相关的分泌型糖蛋白。
Mech Dev. 1998 Oct;77(2):173-84. doi: 10.1016/s0925-4773(98)00139-7.
6
The Xenopus dorsalizing factor Gremlin identifies a novel family of secreted proteins that antagonize BMP activities.非洲爪蟾背化因子Gremlin鉴定出一个拮抗骨形态发生蛋白（BMP）活性的新型分泌蛋白家族。
Mol Cell. 1998 Apr;1(5):673-83. doi: 10.1016/s1097-2765(00)80067-2.
7
A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis.一种新型候选肿瘤抑制基因DAN的产物被分泌到培养基中，并抑制DNA合成。
Eur J Cancer. 1997 Oct;33(12):1986-90. doi: 10.1016/s0959-8049(97)00333-x.
8
Differential interaction of the cyclin-dependent kinase (Cdk) inhibitor p27Kip1 with cyclin A-Cdk2 and cyclin D2-Cdk4.细胞周期蛋白依赖性激酶（Cdk）抑制剂p27Kip1与细胞周期蛋白A-Cdk2和细胞周期蛋白D2-Cdk4的差异相互作用。
J Biol Chem. 1997 Oct 10;272(41):25863-72. doi: 10.1074/jbc.272.41.25863.
9
Identification of a new cellular protein that can interact specifically with DAN.
DNA Cell Biol. 1997 Aug;16(8):985-91. doi: 10.1089/dna.1997.16.985.
10
Yeast ubiquitin-like genes are involved in duplication of the microtubule organizing center.酵母泛素样基因参与微管组织中心的复制。
J Cell Biol. 1996 Jun;133(6):1331-46. doi: 10.1083/jcb.133.6.1331.

DA41在v-Ha-ras-3Y1细胞中的过表达导致生长抑制。

Overexpression of DA41 in v-Ha-ras-3Y1 cells causes growth suppression.

作者信息

Ozaki T, Nakamura Y, Hanaoka E, Nakagawara A, Sakiyama S

机构信息

Division of Biochemistry, Chiba Cancer Center Research Institute, Chuoh-ku, Chiba 260-8717, Japan.

出版信息

Jpn J Cancer Res. 2000 Oct;91(10):987-93. doi: 10.1111/j.1349-7006.2000.tb00875.x.

DOI:10.1111/j.1349-7006.2000.tb00875.x
PMID:11050468
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5926252/
Abstract

We have recently found that DA41 exhibits marked homology with mouse PLIC-1, PLIC-2, frog XDRP1 and yeast DSK2. XDRP1 has been shown to be associated with cyclin A, and blocks cell division of frog embryo. In the present study, we examined the biological role(s) of DA41 in mammalian cells by overexpressing it in v-Ha-ras-transformed 3Y1 cells (ras-3Y1). Transfectants which expressed a high level of DA41 mRNA exhibited a decrease in growth rate, a reduction in saturation density, and a suppression of colony formation in soft agar medium. To clarify the molecular mechanism(s) by which DA41 affects cell growth, the effect of DA41 expression on the levels of various cell cycle-regulatory proteins was examined. The forced expression of DA41 gene resulted in a remarkable reduction in CDK2 activity, while the amount of CDK2 did not change. These observations indicate that DA41 is involved in cell cycle regulation in ras-3Y1 cells.

摘要

我们最近发现，DA41与小鼠PLIC-1、PLIC-2、青蛙XDRP1和酵母DSK2具有显著的同源性。XDRP1已被证明与细胞周期蛋白A相关，并能阻断青蛙胚胎的细胞分裂。在本研究中，我们通过在v-Ha-ras转化的3Y1细胞（ras-3Y1）中过表达DA41来研究其在哺乳动物细胞中的生物学作用。表达高水平DA41 mRNA的转染子在生长速率上有所下降，饱和密度降低，并且在软琼脂培养基中的集落形成受到抑制。为了阐明DA41影响细胞生长的分子机制，我们检测了DA41表达对各种细胞周期调节蛋白水平的影响。DA41基因的强制表达导致CDK2活性显著降低，而CDK2的量没有变化。这些观察结果表明，DA41参与了ras-3Y1细胞的细胞周期调控。