Silveira P P, Xavier M H, Souza F H, Manoli L P, Rosat R M, Ferreira M B, Dalmaz C
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Braz J Med Biol Res. 2000 Nov;33(11):1343-50. doi: 10.1590/s0100-879x2000001100013.
Emotional changes can influence feeding behavior. Previous studies have shown that chronically stressed animals present increased ingestion of sweet food, an effect reversed by a single dose of diazepam administered before testing the animals. The aim of the present study was to evaluate the response of animals chronically treated with midazolam and/or submitted to repeated restraint stress upon the ingestion of sweet food. Male adult Wistar rats were divided into two groups: controls and exposed to restraint 1 h/day, 5 days/week for 40 days. Both groups were subdivided into two other groups treated or not with midazolam (0.06 mg/ml in their drinking water during the 40-day treatment). The animals were placed in a lighted area in the presence of 10 pellets of sweet food (Froot loops). The number of ingested pellets was measured during a period of 3 min, in the presence or absence of fasting. The group chronically treated with midazolam alone presented increased ingestion when compared to control animals (control group: 2.0 +/- 0.44 pellets and midazolam group: 3.60 +/- 0.57 pellets). The group submitted to restraint stress presented an increased ingestion compared to controls (control group: 2.0 +/- 0.44 pellets and stressed group: 4.18 +/- 0.58 pellets). Chronically administered midazolam reduced the ingestion in stressed animals (stressed/water group: 4.18 +/- 0.58 pellets; stressed/midazolam group: 3.2 +/- 0.49 pellets). Thus, repeated stress increases appetite for sweet food independently of hunger and chronic administration of midazolam can decrease this behavioral effect.
情绪变化会影响进食行为。先前的研究表明，长期处于应激状态的动物对甜食的摄入量会增加，在对动物进行测试前给予单剂量地西泮可逆转这种效应。本研究的目的是评估长期接受咪达唑仑治疗和/或遭受反复束缚应激的动物对甜食摄入的反应。成年雄性Wistar大鼠分为两组：对照组和每天接受1小时束缚、每周5天、持续40天的应激组。两组又各自细分为另外两组，分别给予或不给予咪达唑仑治疗（在40天的治疗期间，饮水中含有0.06mg/ml的咪达唑仑）。将动物置于有10粒甜食（果味圈圈糖）的明亮区域。在禁食或不禁食的情况下，测量3分钟内摄入的颗粒数量。与对照动物相比，仅长期接受咪达唑仑治疗的组摄入量增加（对照组：2.0±0.44粒；咪达唑仑组：3.60±0.57粒）。与对照组相比，遭受束缚应激的组摄入量增加（对照组：2.0±0.44粒；应激组：4.18±0.58粒）。长期给予咪达唑仑可减少应激动物的摄入量（应激/饮水组：4.18±0.58粒；应激/咪达唑仑组：3.2±0.49粒）。因此，反复应激会独立于饥饿增加对甜食的食欲，长期给予咪达唑仑可降低这种行为效应。
