Fabe J S, Grahn B H, Paterson P G
College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Canada.
Biol Trace Elem Res. 2000 Summer;75(1-3):43-52. doi: 10.1385/BTER:75:1-3:43.
A study was performed to determine the effect of zinc deficiency on the zinc concentration of the retina, lens, and the retinal pigment epithelium and choroid. Weanling, male Sprague-Dawley rats were fed ad libitum modified AIN-93 diets containing 3 mg zinc/kg diet (-Zn; n = 10) for 6 wk. Control animals were pair-fed (+ZnPF; n = 10) or fed ad libitum (+ZnAL; n = 10) diets containing 100 mg zinc/kg diet. At 6 wk, plasma and tibia zinc were measured by flame atomic absorption spectrophotometry to confirm zinc deficiency. The zinc concentration of ocular tissues was measured by inductively coupled plasma-mass spectrometry. Mean (+/- SEM) lens zinc concentration was significantly depressed in the zinc-deficient group as compared to that of pair-fed or ad libitum-fed controls, suggesting that the role of zinc in cataract formation should be investigated. The zinc concentration of total neural retina was preserved in zinc deficiency. Previously reported deterioration of retinal function in zinc deficiency may be the result of a decline in the zinc concentration of a specific cell layer of the retina that cannot be detected on gross analysis of the entire retina.
进行了一项研究，以确定锌缺乏对视网膜、晶状体、视网膜色素上皮和脉络膜锌浓度的影响。将断乳雄性Sprague-Dawley大鼠随意喂食含3毫克锌/千克饲料的改良AIN-93饲料（-Zn；n = 10），持续6周。对照动物成对喂食（+ZnPF；n = 10）或随意喂食（+ZnAL；n = 10）含100毫克锌/千克饲料的饲料。在6周时，通过火焰原子吸收分光光度法测量血浆和胫骨锌，以确认锌缺乏。通过电感耦合等离子体质谱法测量眼组织的锌浓度。与成对喂食或随意喂食的对照组相比，锌缺乏组晶状体锌浓度平均（±SEM）显著降低，这表明应研究锌在白内障形成中的作用。锌缺乏时，总神经视网膜的锌浓度保持不变。先前报道的锌缺乏时视网膜功能恶化可能是由于视网膜特定细胞层锌浓度下降所致，而在对整个视网膜进行大体分析时无法检测到这种下降。
