Kobayashi H, Uematsu K, Hirayama H, Horikoshi K
Japan Marine Science and Technology Center, Deep-Sea Microorganisms Research Group, Yokosuka 237-0061, Japan.
J Bacteriol. 2000 Nov;182(22):6451-5. doi: 10.1128/JB.182.22.6451-6455.2000.
In studies of Pseudomonas putida IH-2000, a toluene-tolerant microorganism, membrane vesicles (MVs) were found to be released from the outer membrane when toluene was added to the culture. These MVs were found to be composed of phospholipids, lipopolysaccharides (LPS), and very low amounts of outer membrane proteins. The MVs also contained a higher concentration of toluene molecules (0.172 +/- 0. 012 mol/mol of lipid) than that found in the cell membrane. In contrast to the wild-type strain, the toluene-sensitive mutant strain 32, which differs from the parent strain in LPS and outer membrane proteins, did not release MVs from the outer membrane. The toluene molecules adhering to the outer membrane are eliminated by the shedding of MVs, and this system appears to serve as an important part of the toluene tolerance system of IH-2000.
在对耐甲苯微生物恶臭假单胞菌IH-2000的研究中，发现向培养物中添加甲苯时，外膜会释放出膜泡（MVs）。这些膜泡由磷脂、脂多糖（LPS）和极少量的外膜蛋白组成。膜泡中甲苯分子的浓度（0.172±0.012摩尔/摩尔脂质）也高于细胞膜中的浓度。与野生型菌株不同，在LPS和外膜蛋白方面与亲本菌株不同的甲苯敏感突变株32不会从外膜释放膜泡。附着在外膜上的甲苯分子通过膜泡的脱落而被清除，该系统似乎是IH-2000甲苯耐受系统的重要组成部分。
