外膜囊泡：生物发生、功能和问题。
Outer Membrane Vesicles: Biogenesis, Functions, and Issues.
机构信息
Quadram Institute Bioscience, Norwich, United Kingdom.
Norwich Medical School, University of East Anglia, Norwich, United Kingdom.
出版信息
Microbiol Mol Biol Rev. 2022 Dec 21;86(4):e0003222. doi: 10.1128/mmbr.00032-22. Epub 2022 Sep 26.
Abstract
This review focuses on nonlytic outer membrane vesicles (OMVs), a subtype of bacterial extracellular vesicles (BEVs) produced by Gram-negative organisms focusing on the mechanisms of their biogenesis, cargo, and function. Throughout, we highlight issues concerning the characterization of OMVs and distinguishing them from other types of BEVs. We also highlight the shortcomings of commonly used methodologies for the study of BEVs that impact the interpretation of their functionality and suggest solutions to standardize protocols for OMV studies.
摘要
本综述重点介绍了非裂解性外膜囊泡（OMVs），这是革兰氏阴性菌产生的细菌细胞外囊泡（BEVs）的一个亚类，重点介绍了它们的生物发生、货物和功能的机制。在整个过程中，我们强调了与 OMV 表征以及将其与其他类型的 BEVs 区分开来的相关问题。我们还强调了常用于 BEV 研究的方法学的缺点，这些缺点会影响对其功能的解释，并提出了标准化 OMV 研究方案的解决方案。
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