Voisin C, Aerts C, Tonnel A B, Houdret J L, Ramon P
Pathol Biol (Paris). 1975 Jun;23(6):453-9.
Alveolar macrophages of guinea pigs laid on a porous membrane applied to the surface of a reservior filled with nutrient fluid, may be maintained in survival in gaseous phase. The addition of biological material obtained by centrifugation of the broncho-alveolar nutrient fluid, creates in vitro a micro-environment comparable to the physiological alveolar or bronchial environment. Examination of these macrophages under the light and electron microscopes, and study of their phagocytic and bactericidal activity, demonstrate the value of this experimental model, which opens new possibilities for research on the means of phagocytic defence of the respiratory apparatus and their disturbances by gases which pollute the atmosphere.
将豚鼠的肺泡巨噬细胞置于多孔膜上，该膜覆盖在装有营养液的容器表面，这些细胞可在气相中存活。添加通过对支气管肺泡营养液进行离心获得的生物材料，可在体外营造出一种类似于生理肺泡或支气管环境的微环境。利用光学显微镜和电子显微镜对这些巨噬细胞进行检查，并研究它们的吞噬和杀菌活性，证明了该实验模型的价值，它为研究呼吸器官吞噬防御手段以及大气污染气体对其干扰开辟了新的研究途径。
