Voisin C, Aerts C, Jakubczk E, Tonnel A B
Bull Eur Physiopathol Respir. 1977 Jan-Feb;13(1):69-82.
Alveolar macrophages, collected by bronchopulmonary lavage and deposited on a membrane filter applied to the surface of a reservoir filled with nutrient medium, were maintained alive in direct contact with the atmosphere. Studies of morphology (using both optic and electron microscopy), of bactericidal activity and of ATP content confirmed the viability and vitality of the cell culture. This new experimental technique permits the in vitro reconstitution of the alveolar and bronchial microenvironment and offers a new method for the study of the cytotoxicity of toxic gases.
通过支气管肺泡灌洗收集并沉积在应用于充满营养培养基的储液器表面的膜过滤器上的肺泡巨噬细胞，在与大气直接接触的情况下保持存活。形态学研究（使用光学显微镜和电子显微镜）、杀菌活性研究和ATP含量研究证实了细胞培养物的活力和生命力。这种新的实验技术允许在体外重建肺泡和支气管微环境，并为研究有毒气体的细胞毒性提供了一种新方法。
