Fernández-Torres B, Vázquez-Veiga H, Llovo X, Pereiro M, Guarro J
Unitat de Microbiologia, Facultat de Medicina i Institut d'Estudis Avançats, Universitat Rovira i Virgili, Reus, España.
Chemotherapy. 2000 Nov-Dec;46(6):390-4. doi: 10.1159/000007319.
A reference method for dermatophyte in vitro susceptibility testing is lacking. With the advent of new antimycotics, susceptibility testing has received increasing attention as an important laboratory tool for aiding the selection of appropriate drug therapy.
One hundred strains of Trichophyton rubrum were tested against four antifungal agents, itraconazole, clotrimazole, ketoconazole and terbinafine, by using a modification of the proposed standard M38-P of the National Committee for Clinical Laboratory Standards and two types of standardized inocula, 1.4 x 10(4) and 5 x 10(3) CFU/ml.
Terbinafine was revealed to be the most effective antifungal drug. Of the three azole derivatives tested, clotrimazole showed the highest antifungal activity, while the minimum inhibitory concentrations (MICs) of itraconazole and ketoconazole were similar. Inoculum size did not affect the MIC of any of the antifungal agents tested.
Our preliminary data provide promising results for the development of a reference method for dermatophyte susceptibility testing based on the microdilution technique, although more dermatophytes should be tested and the method evaluated in different laboratories.
目前缺乏皮肤癣菌体外药敏试验的参考方法。随着新型抗真菌药物的出现，药敏试验作为辅助选择合适药物治疗的重要实验室工具，受到了越来越多的关注。
采用美国国家临床实验室标准委员会提议的标准M38 - P的改良方法，使用两种标准化接种物，即1.4×10⁴和5×10³CFU/ml，对100株红色毛癣菌进行了4种抗真菌药物的测试，这4种药物分别为伊曲康唑、克霉唑、酮康唑和特比萘芬。
特比萘芬被证明是最有效的抗真菌药物。在所测试的三种唑类衍生物中，克霉唑显示出最高的抗真菌活性，而伊曲康唑和酮康唑的最低抑菌浓度（MIC）相似。接种量不影响所测试的任何一种抗真菌药物的MIC。
我们的初步数据为基于微量稀释技术开发皮肤癣菌药敏试验的参考方法提供了有前景的结果，尽管应该测试更多的皮肤癣菌，并在不同实验室对该方法进行评估。
