Chou P, Lu D W, Chen J T
Department of Ophthalmology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Ophthalmologica. 2000;214(6):421-5. doi: 10.1159/000027536.
In this study, we evaluate the effect of bilateral superior cervical ganglionectomy on albino rabbit choroidal blood flow (CBF) in changes of perfusion pressure (PP). Twenty albino rabbits of either sex weighing between 2.0 and 3.0 kg were randomly divided into two groups. The experimental group (group S) included 10 rabbits (20 eyes) that received bilateral superior cervical ganglionectomy one week prior to the study. The other 10 rabbits (20 eyes) served as a control (group N): each received the same procedure as group S except that the superior cervical ganglion was preserved. By means of a laser Doppler flowmeter (Perimed PF4001), blood cell flux (PF), velocity (V), and concentration of moving blood cells (CMBC) were recorded simultaneously while intraocular pressure was increased linearly by a syringe pump. Blood pressure and intraocular pressure were continuously monitored. The laser beam focused on the posterior pole away from major retinal vessels. When PP decreased from 75 to 0 mm Hg, in group N, PF, V and CMBC decreased from 100% to 6.94+/-0.91%, 8.41+/-0.87%, and 19.38+/-1.11%; in group S, it was 17.75+/-2.58%, 16.78+/-1.48%, and 34.58+/-4.42%, respectively. Group S poses higher PF, V, and CMBC than group N while PP decreased gradually. These results indicate that the superior cervical ganglion plays a role in CBF regulation. Bilateral sympathectomy led to a higher PF in the group S rabbits, indicating increased CBF. PF, V, and CMBC remained constant until PP <55 mm Hg demonstrated the existence of CBF autoregulation within a limited perfusion range in both groups. This autoregulation did not change after sympathectomy.
在本研究中，我们评估双侧颈上神经节切除术对白化兔脉络膜血流（CBF）在灌注压（PP）变化方面的影响。20只体重在2.0至3.0千克之间的雌雄白化兔被随机分为两组。实验组（S组）包括10只兔子（20只眼），在研究前一周接受双侧颈上神经节切除术。另外10只兔子（20只眼）作为对照组（N组）：每只兔子接受与S组相同的手术操作，但保留颈上神经节。通过激光多普勒血流仪（Perimed PF4001），在通过注射泵使眼压线性升高的同时，同步记录血细胞通量（PF）、速度（V）和移动血细胞浓度（CMBC）。持续监测血压和眼压。激光束聚焦于远离主要视网膜血管的后极部。当PP从75毫米汞柱降至0毫米汞柱时，在N组中，PF、V和CMBC分别从100%降至6.94±0.91%、8.41±0.87%和19.38±1.11%；在S组中，分别为17.75±2.58%、16.78±1.48%和34.58±4.42%。随着PP逐渐降低，S组的PF、V和CMBC高于N组。这些结果表明颈上神经节在CBF调节中起作用。双侧交感神经切除术使S组兔子的PF更高，表明CBF增加。在两组中，直到PP<55毫米汞柱时PF、V和CMBC保持恒定，这表明在有限的灌注范围内存在CBF自身调节。这种自身调节在交感神经切除术后没有改变。
