一种新型弯曲杆菌细胞毒素的检测

Detection of a novel campylobacter cytotoxin.

作者信息

Lee A, Smith S C, Coloe P J

机构信息

Department of Applied Biology and Biotechnology, RMIT University, Melbourne and School of Health Sciences, Deakin University, Burwood, Victoria, Australia.

出版信息

J Appl Microbiol. 2000 Oct;89(4):719-25. doi: 10.1046/j.1365-2672.2000.01175.x.

DOI:10.1046/j.1365-2672.2000.01175.x

PMID:11054178

Abstract

The culture filtrates from 10 Campylobacter species were screened for the presence of cytotoxins on a variety of selected tissue culture cell lines. Some Campylobacter jejuni strains showed no effects on tissue culture cell lines compared with other C. jejuni strains, especially C. jejuni 81116, which consistently produced a cytotoxin that was lethal to tissue culture cells. It was observed that CHO cells were the most sensitive cell line in detecting campylobacter cytotoxins. Samples containing the culture filtrate of C. jejuni 81116 prepared at various growth stages were used to determine the subcellular location of the cytotoxin. This C. jejuni 81116 cytotoxin appears to be a heat-stable toxin that is secreted from the cell during stationary phase; cytotoxin activity can be abolished with proteolytic enzymes.

摘要

对10种弯曲杆菌属菌种的培养滤液进行筛选，以检测其在多种选定的组织培养细胞系上是否存在细胞毒素。与其他空肠弯曲菌菌株相比，一些空肠弯曲菌菌株对组织培养细胞系没有影响，尤其是空肠弯曲菌81116，它始终产生一种对组织培养细胞具有致死性的细胞毒素。据观察，CHO细胞是检测弯曲杆菌细胞毒素最敏感的细胞系。使用在不同生长阶段制备的含有空肠弯曲菌81116培养滤液的样品来确定细胞毒素的亚细胞定位。这种空肠弯曲菌81116细胞毒素似乎是一种热稳定毒素，在稳定期从细胞中分泌出来；细胞毒素活性可被蛋白水解酶消除。