Mizuno K, Takama K, Suzuki S
Department of Food Science and Technology, Faculty of Fisheries, Hokkaido University, Japan.
Microbios. 1994;78(317):215-28.
The cytotoxic activity against cultured cells of nineteen clinically isolated strains of Campylobacter jejuni was tested. These strains were found to have different profiles in cytotoxin and enterotoxin production, and the characteristics of cytotoxin were further investigated. The cytotoxin showed cell killing toxicity against CHO and HeLa cells. Vacuole formation was observed in the case of rat hepatocyte primary culture. Treatment with trypsin at 80 degrees C for 30 min inactivated the cytotoxin activity, suggesting that the toxin was protein. The toxin was produced in the culture supernatant with high specific activity per protein, followed by polymyxin and CHAPS treatment fractions in this order. This suggests that the cytotoxin was a cell-releasing toxin and that the active toxin was present as a membrane-associated form. The cytotoxin activity was separated from the enterotoxin activity by DEAE-cellulose column chromatography. The washed fraction contained enterotoxin and cytotoxin, whereas the KCl eluted fraction showed mainly cytotoxic activity.
对19株临床分离的空肠弯曲菌菌株针对培养细胞的细胞毒性活性进行了测试。发现这些菌株在细胞毒素和肠毒素产生方面具有不同的特征，并对细胞毒素的特性进行了进一步研究。该细胞毒素对CHO和HeLa细胞表现出细胞杀伤毒性。在大鼠肝细胞原代培养中观察到空泡形成。在80℃下用胰蛋白酶处理30分钟可使细胞毒素活性失活，表明该毒素是蛋白质。该毒素在培养上清液中产生，每蛋白质具有高比活性，其次是多粘菌素和CHAPS处理级分，顺序依次如此。这表明细胞毒素是一种细胞释放毒素，并且活性毒素以膜相关形式存在。通过DEAE-纤维素柱色谱法将细胞毒素活性与肠毒素活性分离。洗涤级分含有肠毒素和细胞毒素，而KCl洗脱级分主要表现出细胞毒性活性。
