Capel M S, Kjeldgaard M, Engelman D M, Moore P B
Department of Chemistry, Yale University, New Haven, CT 06511.
J Mol Biol. 1988 Mar 5;200(1):65-87. doi: 10.1016/0022-2836(88)90334-8.
Neutron scattering distance data are presented for 33 protein pairs in the 30 S ribosomal subunit from Escherichia coli, along with the methods used for measuring distances between its exchangeable components. When combined with prior data, these new results permit the positioning of S2, S13, S16, S17, S19 and S21 in the 30 S ribosomal subunit, completing the mapping of its proteins by neutron scattering. Comparisons with other data suggest that the neutron map is a reliable guide to the quaternary structure of the 30 S subunit.
本文给出了来自大肠杆菌30S核糖体亚基中33对蛋白质的中子散射距离数据,以及用于测量其可交换成分之间距离的方法。当与先前的数据相结合时,这些新结果能够确定S2、S13、S16、S17、S19和S21在30S核糖体亚基中的位置,从而通过中子散射完成其蛋白质的图谱绘制。与其他数据的比较表明,中子图谱是30S亚基四级结构的可靠指南。