Sonenberg N, Wilchek M, Zamir A
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4332-6. doi: 10.1073/pnas.72.11.4332.
A photolyzable derivative of dipeptidyl-tRNA, p-azido-N-tBoc-Phe-[3H]Phe-tRNA, bound reversibly to 70S ribosomes in the presence of poly(U), becomes, when irradiated, covalently attached to components of the 50S ribosomal subunit. Most of the reaction occurs within the 23S RNA, while ribosomal proteins are only weakly labeled. Reversible binding as well as the covalent reaction are reduced in the absence of poly(U) or in the presence of several antibiotics specific for the 50S ribosomal subunit. There are apparently few sites (or perhaps a single site) of reaction on the 23S RNA that are exclusively located within the 2000 nucleotides from the 3 terminus of the molecule. Part of the 23S RNA within this region must therefore be closely associated with the peptidyl transferase center of the ribosome.
二肽基 - tRNA的一种可光解衍生物,对叠氮基 - N - 叔丁氧羰基 - 苯丙氨酸 - [³H]苯丙氨酸 - tRNA,在聚尿苷酸(poly(U))存在的情况下可逆地结合到70S核糖体上,当受到照射时,会共价连接到50S核糖体亚基的组分上。大部分反应发生在23S RNA内,而核糖体蛋白仅被微弱标记。在没有聚尿苷酸或存在几种对50S核糖体亚基特异的抗生素时,可逆结合以及共价反应都会减少。23S RNA上显然只有很少的反应位点(或者可能是单个位点)专门位于分子3'末端的2000个核苷酸范围内。因此,该区域内的部分23S RNA必定与核糖体的肽基转移酶中心紧密相关。