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位于肽基转移酶中心的23S核糖体RNA上一个位点的鉴定。

Identification of a site on 23S ribosomal RNA located at the peptidyl transferase center.

作者信息

Barta A, Steiner G, Brosius J, Noller H F, Kuechler E

出版信息

Proc Natl Acad Sci U S A. 1984 Jun;81(12):3607-11. doi: 10.1073/pnas.81.12.3607.

DOI:10.1073/pnas.81.12.3607
PMID:6374660
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC345267/
Abstract

3-(4'-Benzoylphenyl)propionyl[3H] Phe-tRNA bound to the peptidyl site of the ribosome is photo-crosslinked exclusively to 23S RNA on irradiation at 320 nm. The site of reaction has been identified both by hybridization and primer-extension experiments as uridine-2584 and uridine-2585, located within the central loop of domain V according to the secondary structure model of 23S RNA. The fact that the covalently crosslinked tRNA retains its ability to form a peptide bond, together with the proximity of this site to the position of several mutations leading to chloramphenicol or erythromycin resistance strongly argue that this region of the 23S-like rRNAs is an integral component of the peptidyl transferase site. On the basis of these results, and from comparative analysis of the 16 available large subunit rRNA sequences, we propose a model for the functional organization of the peptidyl transferase site involving interaction of domains II and V of 23S rRNA.

摘要

与核糖体肽基位点结合的3-(4'-苯甲酰苯基)丙酰基[3H]苯丙氨酰 - tRNA在320nm光照下仅与23S RNA发生光交联。通过杂交和引物延伸实验已确定反应位点为尿苷-2584和尿苷-2585，根据23S RNA的二级结构模型，它们位于结构域V的中央环内。共价交联的tRNA保留其形成肽键的能力，以及该位点与导致氯霉素或红霉素抗性的几个突变位置接近，这有力地表明23S样rRNA的这个区域是肽基转移酶位点的一个组成部分。基于这些结果，并通过对16个可用的大亚基rRNA序列的比较分析，我们提出了一个肽基转移酶位点功能组织的模型，该模型涉及23S rRNA的结构域II和V的相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a132/345267/7d06b99075b6/pnas00613-0010-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a132/345267/43400d1d1cb2/pnas00613-0008-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a132/345267/96a7d2b8ec3b/pnas00613-0008-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a132/345267/b29fbe721efb/pnas00613-0009-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a132/345267/7d06b99075b6/pnas00613-0010-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a132/345267/43400d1d1cb2/pnas00613-0008-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a132/345267/96a7d2b8ec3b/pnas00613-0008-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a132/345267/b29fbe721efb/pnas00613-0009-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a132/345267/7d06b99075b6/pnas00613-0010-a.jpg

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本文引用的文献

1
Altered ribosomal RNA genes in mitochondria from mammalian cells with chloramphenicol resistance.来自具有氯霉素抗性的哺乳动物细胞的线粒体中核糖体RNA基因的改变。
Nature. 1981 Apr 16;290(5807):607-8. doi: 10.1038/290607a0.
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Structure, assembly, and function of ribosomes.核糖体的结构、组装及功能
Curr Top Microbiol Immunol. 1982;97:81-155. doi: 10.1007/978-3-642-68318-3_3.
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Secondary structure model for 23S ribosomal RNA.23S核糖体RNA的二级结构模型
Acta Pharm Sin B. 2014 Aug;4(4):258-65. doi: 10.1016/j.apsb.2014.06.012. Epub 2014 Jul 31.
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Revisiting the structures of several antibiotics bound to the bacterial ribosome.重新审视几种与细菌核糖体结合的抗生素的结构。
Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17158-63. doi: 10.1073/pnas.1008685107. Epub 2010 Sep 27.
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Evolution of protein synthesis from an RNA world.从 RNA 世界到蛋白质合成的进化。
Cold Spring Harb Perspect Biol. 2012 Apr 1;4(4):a003681. doi: 10.1101/cshperspect.a003681.
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RNomics and Modomics in the halophilic archaea Haloferax volcanii: identification of RNA modification genes.嗜盐古菌沃氏嗜盐栖热菌中的RNA组学和修饰组学：RNA修饰基因的鉴定
BMC Genomics. 2008 Oct 9;9:470. doi: 10.1186/1471-2164-9-470.
7
Toward ribosomal RNA catalytic activity in the absence of protein.在无蛋白质情况下实现核糖体RNA的催化活性。
J Mol Evol. 2007 Apr;64(4):472-83. doi: 10.1007/s00239-006-0211-y. Epub 2007 Apr 5.
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The interaction networks of structured RNAs.结构化RNA的相互作用网络。
Nucleic Acids Res. 2006;34(22):6587-604. doi: 10.1093/nar/gkl963. Epub 2006 Nov 28.
9
Solid phase synthesis and binding affinity of peptidyl transferase transition state mimics containing 2'-OH at P-site position A76.在P位点A76位置含有2'-羟基的肽基转移酶过渡态模拟物的固相合成及结合亲和力
Nucleic Acids Res. 2004 Mar 3;32(4):1502-11. doi: 10.1093/nar/gkh311. Print 2004.
10
Preparation and activity of synthetic unmodified mammalian tRNAi(Met) in initiation of translation in vitro.合成未修饰的哺乳动物起始甲硫氨酸转运RNA（tRNAi(Met)）在体外翻译起始中的制备及活性
RNA. 2001 Oct;7(10):1496-505. doi: 10.1017/s135583820101038x.
Nucleic Acids Res. 1981 Nov 25;9(22):6167-89. doi: 10.1093/nar/9.22.6167.
4
Comparison of the structures of free and ribosome-bound tRNAPhe by using slow tritium exchange.利用慢速氚交换比较游离型和核糖体结合型苯丙氨酸转运核糖核酸（tRNAPhe）的结构
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Mitochondrial DNA of chloramphenicol-resistant mouse cells contains a single nucleotide change in the region encoding the 3' end of the large ribosomal RNA.对氯霉素具有抗性的小鼠细胞的线粒体DNA在编码大核糖体RNA 3'端的区域存在一个单核苷酸变化。
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6
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Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
10
Part of the 23S RNA located in the 11S RNA fragment is a constituent of the ribosomal peptidyltransferase centre.位于11S RNA片段中的23S RNA的一部分是核糖体肽基转移酶中心的一个组成部分。
FEBS Lett. 1983 Nov 14;163(2):319-23. doi: 10.1016/0014-5793(83)80844-8.