从膨胀活性污泥中分离出的艾氏021N型丝状菌的系统发育分析及寡核苷酸探针开发
Phylogenetic analysis of and oligonucleotide probe development for eikelboom type 021N filamentous bacteria isolated from bulking activated sludge.
作者信息
Kanagawa T, Kamagata Y, Aruga S, Kohno T, Horn M, Wagner M
机构信息
National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Tsukuba 305-8566, Japan.
出版信息
Appl Environ Microbiol. 2000 Nov;66(11):5043-52. doi: 10.1128/AEM.66.11.5043-5052.2000.
Abstract
Fifteen filamentous strains, morphologically classified as Eikelboom type 021N bacteria, were isolated from bulking activated sludges. Based on comparative 16S ribosomal DNA (rDNA) sequence analysis, all strains form a monophyletic cluster together with all recognized Thiothrix species (88.3 to 98.7% 16S rDNA sequence similarity) within the gamma-subclass of Proteobacteria. The investigated Eikelboom type 021N isolates were subdivided into three distinct groups (I to III) demonstrating a previously unrecognized genetic diversity hidden behind the uniform morphology of the filaments. For in situ detection of these bacteria, 16S rRNA-targeted oligonucleotide probes specific for the entire Eikelboom type 021N-Thiothrix cluster and the Eikelboom type 021N groups I, II, and III, respectively, were designed, evaluated, and successfully applied in activated sludge.
摘要
从膨胀活性污泥中分离出15株丝状菌株，其形态学上归类为艾氏021N型细菌。基于16S核糖体DNA（rDNA）序列的比较分析，所有菌株与变形菌门γ-亚纲内所有已确认的硫丝菌属物种（16S rDNA序列相似度为88.3%至98.7%）共同形成一个单系簇。所研究的艾氏021N型分离株被细分为三个不同的组（I至III），这表明在丝状菌形态一致的背后隐藏着一种先前未被认识到的遗传多样性。为了对这些细菌进行原位检测，分别针对整个艾氏021N-硫丝菌簇以及艾氏021N型I、II和III组设计、评估并成功应用了靶向16S rRNA的寡核苷酸探针于活性污泥中。
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