Mizushima K, Miyamoto Y, Tsukahara F, Hirai M, Sakaki Y, Ito T
Division of Genome Biology, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-0934, Japan.
Genomics. 2000 Nov 1;69(3):314-21. doi: 10.1006/geno.2000.6340.
Differential display screening for region-specific transcripts in rat brain revealed a novel striatum-specific transcript encoding an orphan G-protein-coupled receptor (GPCR) designated Strg/Gpr88 for striatum-specific GPCR. We isolated its homologues from human (HGMW-approved symbol GPR88) and mouse and mapped them to chromosomes 1p21.3 and 3G1, respectively. These loci are syntenic to each other, thereby suggesting their orthology. The predicted primary sequences of Strg/Gpr88 proteins are highly conserved between human and rodents and show the highest level of homology to receptors for biogenic amines. However, Strg/Gpr88 lacks some residues conserved in all known biogenic amine receptors and hence may represent a novel subtype of GPCR. Northern blot and in situ hybridization analyses revealed that Strg/Gpr88 transcripts are expressed almost exclusively in striatum in both human and rodents. Remarkable conservation in primary structure and a unique expression pattern may indicate a role for Strg/Gpr88 in the fundamental functions of striatum such as the control of motor behavior.
通过差异显示筛选大鼠脑中区域特异性转录本，发现了一种新的纹状体特异性转录本，它编码一种孤儿G蛋白偶联受体（GPCR），命名为Strg/Gpr88（纹状体特异性GPCR）。我们从人（HGMW批准符号GPR88）和小鼠中分离出其同源物，并分别将它们定位到染色体1p21.3和3G1上。这些基因座彼此同线，因此表明它们具有直系同源性。Strg/Gpr88蛋白的预测一级序列在人和啮齿动物之间高度保守，并且与生物胺受体具有最高水平的同源性。然而，Strg/Gpr88缺乏在所有已知生物胺受体中保守的一些残基，因此可能代表GPCR的一种新亚型。Northern印迹和原位杂交分析表明，Strg/Gpr88转录本在人和啮齿动物的纹状体中几乎只表达。一级结构的显著保守性和独特的表达模式可能表明Strg/Gpr88在纹状体的基本功能如运动行为控制中起作用。
