Jung B P, Nguyen T, Kolakowski L F, Lynch K R, Heng H H, George S R, O'Dowd B F
Department of Pharmacology, University of Toronto, Ontario, Canada.
Biochem Biophys Res Commun. 1997 Jan 3;230(1):69-72. doi: 10.1006/bbrc.1996.5828.
We amplified human genomic DNA by the polymerase chain reaction (PCR) using oligonucleotides based on the primary sequence of the genes encoding the somatostatin receptors (SSTR) and the somatostatin-like receptor gene SLC-1. One resultant DNA fragment was used to screen a genomic DNA library resulting in the isolation of a gene, GPR25, encoding an additional member of the G protein-coupled receptor family (GPCR). GPR25 is intronless throughout its open reading frame (ORF) and encodes a protein of 360 amino acids. The receptor encoded by GPR25 shares highest identity to the receptor encoded by GPR15, angiotensin II type 1A receptor, and somatostatin receptor 5. Northern analysis found no transcripts expressed in liver or any of the 12 brain regions analyzed. Fluorescence in situ hybridization analysis localized GPR25 to chromosome 1q32.1.
我们使用基于编码生长抑素受体（SSTR）和类生长抑素受体基因SLC-1的基因一级序列的寡核苷酸，通过聚合酶链反应（PCR）扩增人基因组DNA。一个所得的DNA片段用于筛选基因组DNA文库，从而分离出一个基因GPR25，它编码G蛋白偶联受体家族（GPCR）的另一个成员。GPR25在其整个开放阅读框（ORF）中无内含子，编码一个由360个氨基酸组成的蛋白质。GPR25编码的受体与GPR15编码的受体、血管紧张素II 1A型受体和生长抑素受体5具有最高的同源性。Northern分析发现在肝脏或所分析的12个脑区中的任何一个中均无转录本表达。荧光原位杂交分析将GPR25定位到染色体1q32.1。
