环磷酸腺苷 - 鸟苷酸交换因子II（cAMP - GEFII）是环磷酸腺苷（cAMP）在调节性胞吐作用中的直接靶点。
cAMP-GEFII is a direct target of cAMP in regulated exocytosis.
作者信息
Ozaki N, Shibasaki T, Kashima Y, Miki T, Takahashi K, Ueno H, Sunaga Y, Yano H, Matsuura Y, Iwanaga T, Takai Y, Seino S
机构信息
Department of Molecular Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.
出版信息
Nat Cell Biol. 2000 Nov;2(11):805-11. doi: 10.1038/35041046.
Although cAMP is well known to regulate exocytosis in many secretory cells, its direct target in the exocytotic machinery is not known. Here we show that cAMP-GEFII, a cAMP sensor, binds to Rim (Rab3-interacting molecule, Rab3 being a small G protein) and to a new isoform, Rim2, both of which are putative regulators of fusion of vesicles to the plasma membrane. We also show that cAMP-GEFII, through its interaction with Rim2, mediates cAMP-induced, Ca2+-dependent secretion that is not blocked by an inhibitor of cAMP-dependent protein kinase (PKA). Accordingly, cAMP-GEFII is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-independent exocytosis.
尽管环磷酸腺苷（cAMP）在许多分泌细胞中调节胞吐作用已广为人知，但其在胞吐机制中的直接靶点尚不清楚。在此我们表明，一种cAMP传感器cAMP-GEFII与Rim（Rab3相互作用分子，Rab3是一种小G蛋白）以及一种新的异构体Rim2结合，这两者均为囊泡与质膜融合的假定调节因子。我们还表明，cAMP-GEFII通过与Rim2相互作用，介导cAMP诱导的、Ca2+依赖的分泌，而这种分泌不受cAMP依赖性蛋白激酶（PKA）抑制剂的阻断。因此，cAMP-GEFII是调节性胞吐作用中cAMP的直接靶点，并且负责cAMP依赖的、PKA非依赖的胞吐作用。
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