Madeira S, Opromolla D V, Belone A d
Equipe Técnica de Microbiologia, Instituto Lauro de Souza Lima, Bauru, SP, Brasil.
Rev Inst Med Trop Sao Paulo. 2000 Sep-Oct;42(5):239-43. doi: 10.1590/s0036-46652000000500001.
In a previous study, the authors inoculated Swiss mice with Lacazia loboi (L. loboi) and succeeded in maintaining a granulomatous infiltrate and viable fungal cells up to one year and six months after inoculation. Considering the experimental work on paracoccidioidomycosis, 0.03 ml of a fungal suspension obtained from a biopsy of a Jorge Lobo's Disease patient were inoculated into both hind foot pads of 32 six week-old BALB/c mice of both sexes. The animals were sacrificed 1, 4, 7 and 10 months post inoculation. The suspension contained 1.3 x 10(6) fungi/ml and presented 38% viability. Seven months after inoculation, most of the animals presented profuse infiltrates consisting of isolated histiocytes, foreign body and Langhans' giant cells and a large number of fungi, most of them viable. Emergence of macroscopic lesions was observed during the 8th month. Based on fungal count, viability index before and after inoculation, presence of macroscopic lesions and histopathological findings similar to the findings in humans, the authors believe that BALB/c mice may be a good experimental model to study Jorge Lobo's Disease, mainly regarding therapeutic evaluation.
在之前的一项研究中，作者用罗博隐球菌（L. loboi）接种瑞士小鼠，并成功在接种后一年零六个月维持了肉芽肿浸润和存活的真菌细胞。考虑到副球孢子菌病的实验工作，将从一名约热·洛博氏病患者活检中获得的0.03 ml真菌悬液接种到32只六周龄的雌雄BALB/c小鼠的双侧后足垫中。在接种后1、4、7和10个月处死动物。该悬液含有1.3×10(6)个真菌/ml，存活率为38%。接种七个月后，大多数动物出现大量浸润，由孤立的组织细胞、异物和朗汉斯巨细胞以及大量真菌组成，其中大多数真菌存活。在第8个月观察到宏观病变的出现。基于真菌计数、接种前后的活力指数、宏观病变的存在以及与人类发现相似的组织病理学发现，作者认为BALB/c小鼠可能是研究约热·洛博氏病的良好实验模型，主要用于治疗评估。
