Reiner E, Sinko G, Skrinjarić-Spoljar M, Simeon-Rudolf V
Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Arh Hig Rada Toksikol. 2000 Mar;51(1):13-8.
This paper presents a protocol for routine assays of human blood cholinesterase activities which separates erythrocytes from plasma by centrifugation and measures acetylcholinesterase activity in unwashed erythrocytes and butyrylcholinesterase activity in the plasma. The recommended substrate for both enzymes is 1.0 mM acetylthiocholine. The protocol is compared with other two recommended protocols for the activity measurements of the two enzymes using the Ellman method. The paper discusses the advantages and disadvantages of each and concludes with a proposal for an international agreement between laboratories for the evaluation of a standardized protocol.
本文介绍了一种用于人体血液胆碱酯酶活性常规检测的方案，该方案通过离心将红细胞与血浆分离，并测定未洗涤红细胞中的乙酰胆碱酯酶活性以及血浆中的丁酰胆碱酯酶活性。两种酶推荐使用的底物均为1.0 mM乙酰硫代胆碱。该方案与另外两种使用埃尔曼方法测定这两种酶活性的推荐方案进行了比较。本文讨论了每种方案的优缺点，并最后提议实验室之间达成一项国际协议，以评估标准化方案。
