羧基甲基化通过控制调节性B亚基的结合来调节蛋白磷酸酶2A。
Carboxyl methylation regulates phosphoprotein phosphatase 2A by controlling the association of regulatory B subunits.
作者信息
Tolstykh T, Lee J, Vafai S, Stock J B
机构信息
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
出版信息
EMBO J. 2000 Nov 1;19(21):5682-91. doi: 10.1093/emboj/19.21.5682.
Abstract
Phosphoprotein phosphatase 2A (PP2A) is a major phosphoserine/threonine protein phosphatase in all eukaryotes. It has been isolated as a heterotrimeric holoenzyme composed of a 65 kDa A subunit, which serves as a scaffold for the association of the 36 kDa catalytic C subunit, and a variety of B subunits that control phosphatase specificity. The C subunit is reversibly methyl esterified by specific methyltransferase and methylesterase enzymes at a completely conserved C-terminal leucine residue. Here we show that methylation plays an essential role in promoting PP2A holoenzyme assembly and that demethylation has an opposing effect. Changes in methylation indirectly regulate PP2A phosphatase activity by controlling the binding of regulatory B subunits to AC dimers.
摘要
蛋白磷酸酶2A（PP2A）是所有真核生物中一种主要的磷酸丝氨酸/苏氨酸蛋白磷酸酶。它已被分离为一种异源三聚体全酶，由一个65 kDa的A亚基组成，该亚基作为36 kDa催化C亚基结合的支架，以及多种控制磷酸酶特异性的B亚基。C亚基在一个完全保守的C末端亮氨酸残基处被特定的甲基转移酶和甲酯酶可逆地甲基酯化。在这里，我们表明甲基化在促进PP2A全酶组装中起关键作用，而去甲基化则具有相反的作用。甲基化的变化通过控制调节性B亚基与AC二聚体的结合间接调节PP2A磷酸酶活性。
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本文引用的文献
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Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo.磷酸蛋白磷酸酶2A催化亚基的羧基甲基化促进其在体内与调节亚基的功能关联。
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Purification of porcine brain protein phosphatase 2A leucine carboxyl methyltransferase and cloning of the human homologue.猪脑蛋白磷酸酶2A亮氨酸羧基甲基转移酶的纯化及人类同源物的克隆。
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A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A.一种蛋白质磷酸酶甲基酯酶（PME-1）是与蛋白质磷酸酶2A的两个无活性突变体稳定结合的几种新型蛋白质之一。
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Methylated C-terminal leucine residue of PP2A catalytic subunit is important for binding of regulatory Balpha subunit.蛋白磷酸酶2A催化亚基的甲基化C末端亮氨酸残基对于调节性Bα亚基的结合很重要。
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