对银杏花粉的IgE免疫反应。

IgE immune response to Ginkgo biloba pollen.

作者信息

Yun Y Y, Ko S H, Park J W, Hong C S

机构信息

Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.

出版信息

Ann Allergy Asthma Immunol. 2000 Oct;85(4):298-302. doi: 10.1016/S1081-1206(10)62533-1.

DOI:10.1016/S1081-1206(10)62533-1

PMID:11061473

Abstract

BACKGROUND

The ginkgo (Ginkgo biloba L.) continues to be planted as a shade tree in preference to other species in Seoul, Korea. The proportion of ginkgo to total shade trees was 43.2% in 1998, but the allergenic characteristics of ginkgo pollen has not been elucidated.

OBJECTIVES

This study was undertaken to obtain information regarding the skin reactivity rate to ginkgo pollen in a population of Korean subjects with respiratory allergy. Possible ginkgo pollen allergens and the cross-reactivity of ginkgo pollen with other prevalent pollens were also examined.

METHODS

Four hundred and forty-seven patients with asthma and/or allergic rhinitis were skin prick tested with extract of ginkgo pollen (1:20 wt/vol). Of these patients, positive skin responders (A/H ratio > or =2+) were selected for ELISA and immunoblot experiments.

RESULTS

A total of 21 patients (4.7%) showed skin reactivity (A/H ratio > or =2+) to ginkgo pollen in the skin prick test. They were also cosensitized to many other tree, grass, and weed pollens. Sixteen (76%) of the 21 positive skin responders showed specific IgE responses to ginkgo pollen in ELISA. In inhibitory ELISA, IgE binding to ginkgo pollen was inhibited by more than 80% by oak, ryegrass, mugwort, and ragweed; and 34% by hop Japanese; and 10% by rBet v 2 at 10 microg/mL. In immunoblot, 10 out of 21 sera (48%) reacted to the 15-kD protein of ginkgo pollen, 9 (43%) to 33-35 kD, and 8 (38%) to 36-38 kD. In inhibitory immunoblot, IgE binding to ginkgo pollen proteins was almost completely inhibited by oak, ryegrass, mugwort and ragweed, but only partially by hop Japanese and rBet v 2.

CONCLUSION

The skin reactivity rate to ginkgo pollen is approximately 4.7% in a population of Korean subjects with respiratory allergy. Since ginkgo pollen has a high cross-reactivity with other prevalent pollens, it could cause clinical symptoms during its pollen season by cross-reacting with the IgE produced in response to other pollens in patients sensitized to multiple pollens.

摘要

背景

在韩国首尔，银杏树（银杏）作为行道树的种植数量仍多于其他树种。1998年，银杏树在行道树总数中的占比为43.2%，但其花粉的致敏特性尚未明确。

目的

本研究旨在获取韩国呼吸道过敏患者群体对银杏花粉皮肤反应率的相关信息。同时，还对银杏花粉可能的过敏原以及银杏花粉与其他常见花粉的交叉反应性进行了检测。

方法

447例哮喘和/或过敏性鼻炎患者接受了银杏花粉提取物（1:20重量/体积）的皮肤点刺试验。其中皮肤反应阳性（A/H比值≥2+）的患者被选入ELISA和免疫印迹实验。

结果

共有21例患者（4.7%）在皮肤点刺试验中对银杏花粉表现出皮肤反应（A/H比值≥2+）。他们还对许多其他树木、草类和杂草花粉产生了共致敏反应。21例皮肤反应阳性患者中有16例（76%）在ELISA中对银杏花粉表现出特异性IgE反应。在抑制性ELISA中，10μg/mL的橡木、黑麦草、艾蒿和豚草可使与银杏花粉结合的IgE抑制率超过80%；日本蛇麻草的抑制率为34%；rBet v 2的抑制率为10%。在免疫印迹实验中，21份血清中有10份（48%）与银杏花粉的15-kD蛋白发生反应，9份（43%）与33-35 kD蛋白反应，8份（38%）与36-38 kD蛋白反应。在抑制性免疫印迹实验中，橡木、黑麦草、艾蒿和豚草几乎完全抑制了与银杏花粉蛋白结合的IgE，而日本蛇麻草和rBet v 2仅部分抑制。