Mock P, Frydman R, Bellet D, Chassin D, Bischof P, Campana A, Bidart J M
Service de Gynécologie Obstétrique, Hôpital Antoine Béclère, Clamart, France.
J Clin Endocrinol Metab. 2000 Oct;85(10):3941-4. doi: 10.1210/jcem.85.10.6925.
The recent development of a specific immunoassay based on monoclonal antibodies directed to chain C and chain A of early placenta insulin-like peptide (EPIL) encoded by the INSL4 gene, has made it possible to demonstrate pro-EPIL peptide expression during normal pregnancy. In the present study, we report on the expression of pro-EPIL peptides in chromosomally abnormal pregnancies, namely trisomy 21 and 18. EPIL peptide levels were measured in amniotic fluid (AF) and maternal serum (MS) from pregnancies with trisomy 21 (n=16) or 18 (n=14) and compared to levels detected in AF and MS from 33 chromosomally normal pregnancies between 12 and 32 weeks of gestation. Pro-EPIL peptide levels were significantly higher in amniotic fluids from T21 than in AF from chromosomally normal pregnancies (mean pro-EPIL levels +/- SEM, 449+/-129.2 ng/mL vs 137+/-29.6 ng/mL, P = 0.0195), whereas there was only a trend towards an increase in pro-EPIL peptide levels in maternal serum. In a limited matched gestational age range (15 to 17 weeks), it was confirmed that pro-EPIL peptide levels were significantly higher in AF from T21 pregnancies (644.0+/-155.9 ng/mL, n = 11) than in AF from normal pregnancies (177.8+/-39.0 ng/mL, n = 12; P < 0.0001). Interestingly, the expression patterns of pro-EPIL peptides, human chorionic gonadotropin (hCG) and its free subunits were parallel in T21 pregnancies as recently observed in normal pregnancies. These results are in line with previous observations suggesting that the biosynthesis of both hCG and EPIL follows common regulation pathways.
基于针对由INSL4基因编码的早期胎盘胰岛素样肽（EPIL）的C链和A链的单克隆抗体开发的特异性免疫测定法，使得在正常妊娠期间证明前EPIL肽的表达成为可能。在本研究中，我们报告了前EPIL肽在染色体异常妊娠中的表达，即21三体和18三体。测量了21三体（n = 16）或18三体（n = 14）妊娠的羊水（AF）和母血清（MS）中的EPIL肽水平，并与妊娠12至32周的33例染色体正常妊娠的AF和MS中检测到的水平进行比较。21三体妊娠的羊水中前EPIL肽水平显著高于染色体正常妊娠的AF（平均前EPIL水平+/- SEM，449 +/- 129.2 ng/mL对137 +/- 29.6 ng/mL，P = 0.0195），而母血清中前EPIL肽水平仅呈上升趋势。在有限的匹配孕周范围内（15至17周），证实21三体妊娠的AF中前EPIL肽水平（644.0 +/- 155.9 ng/mL，n = 11）显著高于正常妊娠的AF（177.8 +/- 39.0 ng/mL，n = 12；P < 0.0001）。有趣的是，正如最近在正常妊娠中观察到的那样，21三体妊娠中前EPIL肽、人绒毛膜促性腺激素（hCG）及其游离亚基的表达模式是平行的。这些结果与先前的观察结果一致，表明hCG和EPIL的生物合成遵循共同的调节途径。
