Bondi M, Messi P, Guerrieri E, Bitonte F
Dipartment of Biomedical Sciences, University of Modena and Reggio E., Italy.
New Microbiol. 2000 Oct;23(4):347-56.
Thirty water isolates of A. hydrophila were tested for potential virulence profiles, antibiotic resistance and Bacteriocin-Like Substances (BLS) production. Cytotoxic activity was present in all strains tested, 87% were hemolytic and 70% adhesive. Lysine decarboxylase reactions (LDC) positivity was correlated with virulence factors: 100% versus cytotoxicity, 84% versus adherence, 76% versus hemolytic activity. The correlation was also present in the LDC-negative strains. Hemolytic and cytotoxic activities were frequently associated: high cytotoxicity, corresponding to high hemolytic activity and vice versa. The in vitro susceptibility of A. hydrophila to 28 antibacterial agents showed that cefotaxime was the most active beta-lactam antibiotic, and Cefuroxime inhibited 90% of the strains. Isolates were resistant to Penicillin G, Ampicillin, Carbenicillin, Amoxicillin, Cephalotin and Cefaclor. Tetracycline, Chloramphenicol, Nitrofurantoine, the quinolones and the aminoglycosides (except Streptomycin) were consistently active. BLS production never emerged against closely-related microorganisms. On the contrary A. hydrophila presented a heteroinhibitory activity against non-taxonomically related genera such as Listeria spp. (L. seeligeri NCTC 11856, L. welshimeri NCTC 11857, L. ivanovii NCTC 11846) and S. aureus ATCC 25923. Although a large number of strains showed virulence determinants together with other biological characters such as antibiotic resistance and BLS production, it was not possible to relate these factors to the observed plasmids.
对30株嗜水气单胞菌的水分离株进行了潜在毒力谱、抗生素耐药性和类细菌素物质（BLS）产生情况的测试。所有测试菌株均具有细胞毒性活性，87%的菌株具有溶血活性，70%的菌株具有黏附性。赖氨酸脱羧酶反应（LDC）阳性与毒力因子相关：细胞毒性方面为100%，黏附方面为84%，溶血活性方面为76%。LDC阴性菌株中也存在这种相关性。溶血活性和细胞毒性活性经常相关：高细胞毒性对应高溶血活性，反之亦然。嗜水气单胞菌对28种抗菌剂的体外敏感性表明，头孢噻肟是最具活性的β-内酰胺抗生素，头孢呋辛抑制了90%的菌株。分离株对青霉素G、氨苄西林、羧苄西林、阿莫西林、头孢噻吩和头孢克洛耐药。四环素、氯霉素、呋喃妥因、喹诺酮类和氨基糖苷类（除链霉素外）始终具有活性。从未发现针对密切相关微生物产生BLS。相反，嗜水气单胞菌对非分类学相关属如李斯特菌属（西利格李斯特菌NCTC 11856、威尔希默李斯特菌NCTC 11857、伊万诺夫李斯特菌NCTC 11846）和金黄色葡萄球菌ATCC 25923呈现异源抑制活性。尽管大量菌株表现出毒力决定因素以及其他生物学特性如抗生素耐药性和BLS产生，但无法将这些因素与观察到的质粒联系起来。
