Messi Patrizia, Guerrieri Elisa, Bondi Moreno
Department of Biomedical Sciences, University of Modena and Reggio E., Via Campi 287, 41100 Modena, Italy.
FEMS Microbiol Lett. 2003 Mar 14;220(1):121-5. doi: 10.1016/S0378-1097(03)00092-2.
30 Aeromonas hydrophila water isolates were tested for bacteriocin-like substance (BLS) production using a target panel of closely related microorganisms and other Gram-positive and Gram-negative bacteria, including food-borne pathogens. A. hydrophila showed antibacterial activity against one or more indicator microorganisms, but the activity emerged only with non-phylogenetically related genera or species. In particular all A. hydrophila showed antibacterial activity against one or more of the tested Staphylococcus strains, five against Listeria spp. (Listeria seeligeri, Listeria welshimeri and Listeria ivanovii), and eight presented a weak antagonistic activity towards Streptococcus agalactiae and Lactobacillus spp. Inhibitory activity was not observed against the other Gram-positive (Listeria monocytogenes, Listeria innocua and Enterococcus spp.) and Gram-negative tested strains, including Aeromonas sobria, Aeromonas caviae and the same A. hydrophila, when used as indicator. Anti-staphylococcal activity was observed with a gradual increase of the inhibition zone during incubation and seemed to be influenced by A. hydrophila hemolytic expression. Extrachromosomal analysis showed the presence, in 70% of the strains, of one to five plasmids with molecular masses ranging from 2.1 to 41.5 MDa, but it was not possible to relate this result with BLS production.
使用一组密切相关的微生物以及其他革兰氏阳性和革兰氏阴性细菌（包括食源性病原体）作为靶标，对30株嗜水气单胞菌水分离株进行了类细菌素物质（BLS）产生情况的检测。嗜水气单胞菌对一种或多种指示微生物表现出抗菌活性，但这种活性仅在非系统发育相关的属或种中出现。特别是，所有嗜水气单胞菌对一种或多种测试的葡萄球菌菌株表现出抗菌活性，五株对李斯特菌属（斯氏李斯特菌、威氏李斯特菌和伊氏李斯特菌）有抗菌活性，八株对无乳链球菌和乳杆菌属表现出微弱的拮抗活性。当用作指示菌时，未观察到对其他革兰氏阳性菌（单核细胞增生李斯特菌、无害李斯特菌和肠球菌属）以及测试的革兰氏阴性菌（包括温和气单胞菌、豚鼠气单胞菌和同一株嗜水气单胞菌）的抑制活性。在培养过程中观察到抗葡萄球菌活性，其抑菌圈逐渐增大，且似乎受嗜水气单胞菌溶血表达的影响。染色体外分析显示，70%的菌株中存在一到五个质粒，分子量范围为2.1至41.5 MDa，但无法将此结果与BLS的产生联系起来。
