Johannsen U, Schoppmeyer K
Arch Exp Veterinarmed. 1975;29(1):33-62.
Broth culture filtrate containing endotoxin, prepared from serotype O 139:K82(?):H1 was given by the intra-enteric route with and without dimethyl sulphoxide, and with or without blockade of the RES by intravenous injection of trypan blue, using about five piglets for each of the four combinations. Clinical signs, blood pressure, ECG, respiration, temperature, haematology and pathological findings were recorded. Coli enterotoxaemia manifested by fatal endotoxin shock developed in all ten piglets given toxin plus dimethyl sulphoxide, and in 4 of 5 similarly treated after RES blockade. The sondrmoe did not develop in piglets given large amounts of toxin without dimethyl sulphoxide, whether the RES was blocked or not. When enteric absorption of toxin was promoted by dimethyl sulphoxide, RES blockade increased the sensitivity of the animal to toxin (shortening of the time till death). The results show that there are two functional barriers to endotoxin: - the intestinal barrier, which normally prevents large amounts of toxin from entering the circulation; and RES, which plays a part in detoxifying and eliminating endotoxin which has been absorbed. Application of these findings to the pathogenesis of coli, enterotoxaemia is discussed.
用血清型O 139:K82（？）:H1制备的含内毒素的肉汤培养滤液，通过肠内途径给予，分别添加和不添加二甲基亚砜，并且通过静脉注射锥虫蓝阻断或不阻断网状内皮系统（RES），四种组合每种大约使用五只仔猪。记录临床症状、血压、心电图、呼吸、体温、血液学和病理学检查结果。在给予毒素加二甲基亚砜的所有十只仔猪以及在RES阻断后进行类似处理的五只仔猪中的四只中，出现了以致命性内毒素休克为表现的大肠杆菌肠毒血症。在不添加二甲基亚砜给予大量毒素的仔猪中，无论RES是否被阻断，均未出现该综合征。当二甲基亚砜促进毒素的肠道吸收时，RES阻断会增加动物对毒素的敏感性（缩短直至死亡的时间）。结果表明，对内毒素存在两种功能性屏障： - 肠道屏障，通常可防止大量毒素进入循环；以及RES，它在对已吸收的内毒素进行解毒和清除中发挥作用。讨论了这些发现应用于大肠杆菌肠毒血症发病机制的情况。
