Wahby A F
Department of Molecular Biology, National Research Center, Dokki, Cairo, Egypt.
Clin Diagn Lab Immunol. 2000 Nov;7(6):915-9. doi: 10.1128/CDLI.7.6.915-919.2000.
A combined cell culture enzyme-linked immunosorbent assay (CCC-ELISA) was developed for measuring the neutralizing antipoliovirus antibodies in human sera. The binding of different concentrations of each of the three poliovirus types to BGM cells in the presence and absence of a constant dilution from each test and reference serum was measured in the CCC-ELISA. The titers of the viruses neutralized by each serum were measured with the titration curves and used for interpretation of neutralizing titers to the three poliovirus types. Analysis of human sera revealed that the sensitivity and specificity of the CCC-ELISA and the microneutralization assay were comparable. The CCC-ELISA is nonsubjective, rapid, and highly reproducible. Furthermore, the CCC-ELISA could potentially be used as a seroepidemiologic tool for assessment of the humoral response to the cell culture infectious viruses.
开发了一种联合细胞培养酶联免疫吸附测定法(CCC-ELISA),用于检测人血清中的中和抗脊髓灰质炎病毒抗体。在CCC-ELISA中,测量了三种脊髓灰质炎病毒各不同浓度在存在和不存在来自每个测试血清和参考血清的恒定稀释液的情况下与BGM细胞的结合。用滴定曲线测量每种血清中和的病毒滴度,并用于解释对三种脊髓灰质炎病毒类型的中和滴度。对人血清的分析表明,CCC-ELISA和微量中和试验的敏感性和特异性相当。CCC-ELISA不主观、快速且具有高度可重复性。此外,CCC-ELISA有可能用作血清流行病学工具,以评估对细胞培养感染性病毒的体液反应。