Ehrenfeld E, Brown D, Jia X Y, Summers D F
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
J Infect Dis. 1995 Apr;171(4):845-50. doi: 10.1093/infdis/171.4.845.
Sera from recent and past cases of poliomyelitis as well as from healthy subjects vaccinated with live attenuated poliovirus, all contain antibodies to poliovirus nonstructural proteins. Reactivity of these antisera with nonstructural protein antigens present in virus-infected HeLa cells or translated from cDNA transcripts in rabbit reticulocyte lysates was sometimes sensitive to denaturation of the antigens. The finding of antibodies to nonstructural proteins of poliovirus and other viruses should have applications in the development of diagnostic assays for virus infection and may have implications for future vaccine design.
近期和既往脊髓灰质炎病例的血清，以及接种了减毒活脊髓灰质炎病毒的健康受试者的血清，均含有针对脊髓灰质炎病毒非结构蛋白的抗体。这些抗血清与病毒感染的HeLa细胞中存在的非结构蛋白抗原或兔网织红细胞裂解物中从cDNA转录本翻译而来的抗原的反应性，有时对抗原变性敏感。脊髓灰质炎病毒和其他病毒非结构蛋白抗体的发现，在病毒感染诊断检测的开发中应具有应用价值，并且可能对未来疫苗设计有影响。
