Inoue T, Thomas J H
Department of Genetics, University of Washington, Seattle, Washington 98195, USA.
Genetics. 2000 Nov;156(3):1035-46. doi: 10.1093/genetics/156.3.1035.
The dauer is a developmentally arrested alternative third larval stage of Caenorhabditis elegans. Entry into this state is regulated by environmental cues, including temperature, food, and the concentration of constitutively secreted dauer pheromone. Genetically, three parallel pathways have been found that regulate this process. Of these, the group 2 pathway, which includes the genes daf-1, daf-3, daf-4, daf-5, daf-7, daf-8, and daf-14, mediates the transduction of environmental signals through the ASI chemosensory neuron and encodes a TGF-beta-related signaling pathway. To identify additional genes that function in this pathway, we carried out a screen for suppressors of mutations in daf-1, daf-8, and daf-14. From the total of 36 mutations, seven complementation groups were identified. Three complementation groups correspond to the previously described genes daf-3, daf-5, and daf-12. Three correspond to novel genes scd-1, scd-2, and scd-3. Genetic analysis of these scd genes is presented here. A fourth complementation group was represented by a single mutation sa315, which affects the daf-2/age-1 insulin-related signaling pathway.
滞育是秀丽隐杆线虫发育停滞的第三种幼虫阶段。进入这种状态受环境线索调节，包括温度、食物和持续分泌的滞育信息素浓度。在遗传学上，已发现三条平行途径调节这一过程。其中，第2组途径包括基因daf-1、daf-3、daf-4、daf-5、daf-7、daf-8和daf-14，通过ASI化学感应神经元介导环境信号的转导，并编码一条与TGF-β相关的信号通路。为了鉴定在该途径中起作用的其他基因，我们对daf-1、daf-8和daf-14突变的抑制子进行了筛选。从总共36个突变中，鉴定出7个互补群。三个互补群对应于先前描述的基因daf-3、daf-5和daf-12。三个对应于新基因scd-1、scd-2和scd-3。本文介绍了这些scd基因的遗传分析。第四个互补群由单个突变sa315代表，它影响daf-2/age-1胰岛素相关信号通路。
