Kayser K, Blum S, Beyer M, Haroske G, Kunze K D, Meyer W
Department of Pathology, Thoraxklinik, Heidelberg, Germany.
J Clin Pathol. 2000 Oct;53(10):760-4. doi: 10.1136/jcp.53.10.760.
To analyse the practicability and potential assistance of static DNA cytometry performed by means of the remote quantitation server Euroquant and the internet in routine diagnostic analysis of pleural effusions, and to outline the role of DNA cytometry on pleural effusions in distinguishing between benign and malignant (and herein primary versus metastatic) effusions.
Cytological smears of 294 pleural effusions were stained with the Feulgen method. The DNA content of a minimum of 300 randomly chosen analysis nuclei and 30 reference nuclei (lymphocytes) was measured by internet connection to the remote quantitation server Euroquant. Cytometric features were derived from the histograms, and the time needed for case evaluation, the reliability of staining and measurement procedures, and the contribution to the final diagnosis were evaluated.
Only 120 of 294 pleural effusions could be measured. The total measurement time for each specimen was 60 minutes. The guidelines of the consensus report on DNA measurements were fulfilled. Seventy eight malignant (18 mesotheliomas, 60 metastatic tumours) and 42 benign effusions were measured. Seven of 78 malignant effusions were euploid and none of 42 benign effusions were aneuploid. The sensitivity and specificity were 91% and 100%, respectively, for distinguishing benign from malignant effusions, and 95% and 100%, respectively, for discriminating between benign and malignant effusions caused by metastatic malignant tumours.
Static DNA cytometry using the remote quantitation server Euroquant can be performed reliably in the routine diagnosis of pleural effusions; however, only 40% of effusions meet the technical requirements for static DNA cytometry. Within the measurable cases, static DNA cytometry made an important contribution to the confirmation/exclusion of malignancy.
分析通过远程定量服务器Euroquant和互联网进行的静态DNA细胞计数在胸腔积液常规诊断分析中的实用性和潜在辅助作用，并概述DNA细胞计数在区分胸腔积液的良性与恶性（以及原发性与转移性）方面的作用。
对294例胸腔积液的细胞学涂片进行福尔根染色。通过与远程定量服务器Euroquant联网，测量至少300个随机选择的分析细胞核和30个参照细胞核（淋巴细胞）的DNA含量。从直方图中得出细胞计数特征，并评估病例评估所需时间、染色和测量程序的可靠性以及对最终诊断的贡献。
294例胸腔积液中只有120例能够进行测量。每个标本的总测量时间为60分钟。满足了DNA测量共识报告的指导原则。测量了78例恶性积液（18例间皮瘤，60例转移性肿瘤）和42例良性积液。78例恶性积液中有7例为整倍体，42例良性积液中无一例为非整倍体。区分良性与恶性积液的敏感性和特异性分别为91%和100%，区分转移性恶性肿瘤所致良性与恶性积液的敏感性和特异性分别为95%和100%。
使用远程定量服务器Euroquant进行静态DNA细胞计数在胸腔积液的常规诊断中可可靠进行；然而，只有40%的积液符合静态DNA细胞计数的技术要求。在可测量的病例中，静态DNA细胞计数对恶性肿瘤的确诊/排除有重要贡献。
