Haroske G, Meyer W, Theissig F, Schubert K, Kunze K D
Institute of Pathology, University of Technology, Dresden, Germany.
Anal Quant Cytol Histol. 1998 Aug;20(4):302-12.
To remotely test a stepwise framework of quality control measures, developed according to the 1997 European Society of Analytical Cellular Pathology consensus on diagnostic DNA image cytometry, in two series of measurements by means of the quantitation server EUROQUANT.
In each of these series, 104 fine needle aspiration biopsies, imprints from breast cancer specimens and 28 rat liver imprints were measured twice at two different cytometry workstations. Further measurements on special rat liver specimens for evaluation of the stability of the machinery and of the preparation process were done at both workstations. Afterwards the measurement data from both machines were transferred to the quantitation server and analyzed automatically. Beside the classical DNA histograms, a set of further evaluations was performed to detect optical errors as well as inhomogeneities in the measurements. Running values for mean integrated optical density values, mean corrective factors and mean coefficients of variations of corrective factors were computed to control the stability of the entire methodology over time.
The study demonstrated the preconditions and outcome of server-based quality control in DNA ploidy analysis. The results show that such a remote analysis is feasible and comparable to local DNA ploidy analysis. It is also demonstrated how quality control tests reflect the process performance of ploidy analysis at its different levels.
Quality control must be an inherent part of the daily routine to allow a reliable diagnostic interpretation and ensure steadily high product quality.
通过定量服务器EUROQUANT，在两组测量中远程测试根据1997年欧洲分析细胞病理学学会关于诊断性DNA图像细胞术的共识制定的质量控制措施逐步框架。
在这两组测量中，分别在两个不同的细胞术工作站对104份细针穿刺活检标本、乳腺癌标本印片和28份大鼠肝脏印片进行了两次测量。在两个工作站对特殊大鼠肝脏标本进行了进一步测量，以评估仪器和制备过程的稳定性。之后，将两台仪器的测量数据传输到定量服务器并进行自动分析。除了经典的DNA直方图外，还进行了一系列进一步评估，以检测测量中的光学误差和不均匀性。计算平均积分光密度值、平均校正因子和校正因子变异系数的运行值，以控制整个方法随时间的稳定性。
该研究证明了基于服务器的DNA倍体分析质量控制的前提条件和结果。结果表明，这种远程分析是可行的，并且与本地DNA倍体分析具有可比性。还展示了质量控制测试如何在不同层面反映倍体分析的过程性能。
质量控制必须成为日常工作的固有组成部分，以实现可靠的诊断解读并确保产品质量持续保持高水平。
