Motherby H, Marcy T, Hecker M, Ross B, Nadjari B, Auer H, Müller K M, Häussinger D, Strauer B E, Böcking A
Institute of Cytopathology, Heinrich Heine University, Düsseldorf, Federal Republic of Germany.
Anal Quant Cytol Histol. 1998 Jun;20(3):153-61.
To determine whether DNA aneuploidy is a sensitive and specific marker for the identification of tumor cells in effusions and whether the pattern of DNA aneuploidy can provide important information for the differential diagnosis of primary and secondary tumors of the serous membranes.
One hundred eight malignant mesotheliomas as well as 102 metastatic carcinomas of the serous membranes were obtained from routine cytologic and histologic material. One hundred reactive effusions were investigated as controls. Nuclear DNA contents were measured after Feulgen staining using a TV image analysis system.
DNA aneuploidy was assumed if abnormal DNA stemlines, a coefficient of variation of the first DNA stemline > or = 10%, or cells > 9c were observed. On this basis the prevalence of DNA aneuploidy in mesotheliomas was 83% for cytologic and 84% for histologic material. In effusions of metastatic carcinomas it was 100%. None of the 100 reactive effusions revealed DNA aneuploidy (prevalence, 0%). Positive predictive value for mesotheliomas was 100%; negative predictive value was 88% for cytologic and 82% for histologic material. Positive predictive value for metastatic carcinomas was 100%; negative predictive value was 100%. Seventy-two percent of the mesotheliomas revealed their greatest stemline within the range 1.80c-2.20c, whereas none of the metastatic carcinomas showed this stemline position.
DNA image cytometry might be a very sensitive and highly specific, additional tool for identification of neoplastic cells in effusions as well as for the differential diagnosis of mesothelioma vs. metastatic carcinoma of the serous membranes.
确定DNA非整倍体是否为识别积液中肿瘤细胞的敏感且特异的标志物，以及DNA非整倍体模式是否可为浆膜原发性和继发性肿瘤的鉴别诊断提供重要信息。
从常规细胞学和组织学材料中获取108例恶性间皮瘤以及102例浆膜转移性癌。将100例反应性积液作为对照进行研究。使用电视图像分析系统在福尔根染色后测量核DNA含量。
若观察到异常DNA干系、第一条DNA干系的变异系数≥10%或细胞>9c，则判定为DNA非整倍体。在此基础上，间皮瘤中DNA非整倍体的细胞学材料患病率为83%，组织学材料患病率为84%。在转移性癌的积液中，患病率为100%。100例反应性积液均未显示DNA非整倍体（患病率，0%）。间皮瘤的阳性预测值为100%；细胞学材料的阴性预测值为88%，组织学材料的阴性预测值为82%。转移性癌的阳性预测值为100%；阴性预测值为100%。72%的间皮瘤显示其最大干系在1.80c - 2.20c范围内，而转移性癌均未显示此干系位置。
DNA图像细胞术可能是一种非常敏感且高度特异的辅助工具，用于识别积液中的肿瘤细胞以及浆膜间皮瘤与转移性癌的鉴别诊断。
