Liu G D, Hu K S, Li W, Shen G, Yu R Q
College of Chemistry and Chemical Engineering, Institute for Chemometrics and Sensing Technology, Hunan University, Changsha, China.
Analyst. 2000 Sep;125(9):1595-9. doi: 10.1039/b004005n.
A renewable electrochemical immunosensor was developed for the determination of transferrin in human serum. It is based on a paraffin-graphite-transferrin antiserum biocomposite, which needs no additional curing. A competitive binding assay was used to determine transferrin in human serum with the aid of transferrin labeled with horseradish peroxidase. The assay conditions were optimized, including the loading of transferrin antiserum in the biocomposite, the amount of labeled transferrin in the incubation solution, incubation time and temperature. Serum samples were analyzed and the results demonstrate that the concentration range of determination with this system meets the demands of clinical analysis. The surface of the immunosensor can be regenerated by simply polishing to obtain a fresh immunocomposite ready to be used in a new competitive assay.
开发了一种可再生电化学免疫传感器用于测定人血清中的转铁蛋白。它基于石蜡-石墨-转铁蛋白抗血清生物复合材料，无需额外固化。采用竞争性结合测定法，借助辣根过氧化物酶标记的转铁蛋白测定人血清中的转铁蛋白。对测定条件进行了优化，包括生物复合材料中转铁蛋白抗血清的负载量、孵育溶液中标记转铁蛋白的量、孵育时间和温度。对血清样本进行了分析，结果表明该系统的测定浓度范围符合临床分析的要求。免疫传感器的表面可以通过简单抛光进行再生，以获得可用于新的竞争性测定的新鲜免疫复合材料。
