Santandreu M, Céspedes F, Alegret S, Martínez-Fàbregas E
Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
Anal Chem. 1997 Jun 1;69(11):2080-5. doi: 10.1021/ac961222b.
Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgC1). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay.
基于刚性生物复合材料构建了新型可抛光免疫传感器。这些生物复合材料包含石墨粉、兔免疫球蛋白G（IgG）以及甲基丙烯酸酯或环氧树脂。这种材料同时作为生物分子的储存库和传感器。为了研究这种新型材料的潜在分析特性，开发了一种竞争性结合测定法，借助碱性磷酸酶标记的山羊抗兔IgG来测定样品中存在的兔IgG。以苯基磷酸为底物，在800 mV（相对于Ag/AgCl）下通过安培法检测酶促反应产生的苯酚。免疫传感器的表面可通过简单抛光进行再生，得到可用于新的竞争性测定的新鲜免疫复合材料。
