Kukuczka S M, Grosso L E
Department of Pathology, St Louis University School of Medicine, St Louis, MO 63104, USA.
Mol Diagn. 2000 Jun;5(2):151-4. doi: 10.1007/BF03262033.
The increasing clinical use of diagnostic DNA mutation analysis requires efficient isolation of DNA from peripheral blood.
The use of a vacuum manifold to isolate DNA was evaluated and compared with a similar centrifugation-based DNA isolation technique. In PCR-based assays of five-point mutations, identical results were obtained with DNA isolated from peripheral blood using either centrifugation or a vacuum system. Minor modifications to PCR procedures were encountered.
In the clinical setting, this vacuum-driven method of DNA isolation provides an efficient, useful alternative to conventional centrifugation-based DNA isolation from peripheral-blood specimens. Providing sufficient, stable DNA for multiple assays, it is easily implemented without highly specialized, expensive equipment and decreases the time spent isolating DNA from multiple samples. In addition, the potential for specimen contamination is reduced because there are fewer transfer steps.
诊断性DNA突变分析在临床中的应用日益增加，这需要从外周血中高效分离DNA。
对使用真空歧管分离DNA进行了评估，并与一种类似的基于离心的DNA分离技术进行了比较。在基于PCR的五点突变检测中，使用离心法或真空系统从外周血中分离的DNA得到了相同的结果。PCR程序需要进行一些小的修改。
在临床环境中，这种真空驱动的DNA分离方法为从外周血标本中进行传统的基于离心的DNA分离提供了一种高效、有用的替代方法。它能为多次检测提供足够、稳定的DNA，易于实施，无需高度专业化、昂贵的设备，还能减少从多个样本中分离DNA所花费的时间。此外，由于转移步骤较少，标本污染的可能性也降低了。
