使用滤纸和Chelex-100对血液标本进行快速有效的诊断性聚合酶链反应处理。
Rapid and effective processing of blood specimens for diagnostic PCR using filter paper and Chelex-100.
作者信息
Polski J M, Kimzey S, Percival R W, Grosso L E
机构信息
Department of Pathology, Saint Louis University Health Sciences Center, MO 63104, USA.
出版信息
Mol Pathol. 1998 Aug;51(4):215-7. doi: 10.1136/mp.51.4.215.
Abstract
AIM
To provide a more efficient method for isolating DNA from peripheral blood for use in diagnostic DNA mutation analysis.
METHODS
The use of blood impregnated filter paper and Chelex-100 in DNA isolation was evaluated and compared with standard DNA isolation techniques.
RESULTS
In polymerase chain reaction (PCR) based assays of five point mutations, identical results were obtained with DNA isolated routinely from peripheral blood and isolated using the filter paper and Chelex-100 method.
CONCLUSION
In the clinical setting, this method provides a useful alternative to conventional DNA isolation. It is easily implemented and inexpensive, and provides sufficient, stable DNA for multiple assays. The potential for specimen contamination is reduced because most of the steps are performed in a single microcentrifuge tube. In addition, this method provides for easy storage and transport of samples from the point of acquisition.
摘要
目的
提供一种更高效的从外周血中分离DNA的方法，用于诊断性DNA突变分析。
方法
评估了在DNA分离过程中使用浸血滤纸和螯合树脂100，并与标准DNA分离技术进行比较。
结果
在基于聚合酶链反应（PCR）的五点突变检测中，从外周血常规分离的DNA以及使用滤纸和螯合树脂100方法分离的DNA得到了相同的结果。
结论
在临床环境中，该方法为传统DNA分离提供了一种有用的替代方法。它易于实施且成本低廉，并为多次检测提供足够、稳定的DNA。由于大多数步骤在单个微量离心管中进行，降低了样本污染的可能性。此外，该方法便于从采集点对样本进行储存和运输。
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